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Harmful phytoplankton diversity and dynamics in an upwelling region (Sagres, SW Portugal) revealed by ribosomal RNA microarray combined with microscopy

2019-02Journal article Restricted access
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dc.contributor.authorDanchenko, Sergei
dc.contributor.authorFragoso, Bruno
dc.contributor.authorGuillebault, Delphine
dc.contributor.authorIcely, John
dc.contributor.authorBerzano, Marco
dc.contributor.authorNewton, Alice
dc.date.accessioned2020-07-24T10:52:52Z
dc.date.available2020-07-24T10:52:52Z
dc.date.issued2019-02
dc.description.abstractThe study region in Sagres, SW Portugal, is subject to natural eutrophication of coastal waters by wind-driven upwelling, which stimulates high primary productivity facilitating the recent economic expansion of bivalve aquaculture in the region. However, this economic activity is threatened by harmful algal blooms (HAB) caused by the diatoms Pseudo-nitzschia spp., Dinophysis spp. and other HAB dinoflagellates, all of which can produce toxins, that can induce Amnesic Shellfish Poisoning (ASP), Diarrhetic Shellfish Poisoning (DSP) and Paralytic Shellfish Poisoning (PSP). This study couples traditional microscopy with 18S/28S rRNA microarray to improve the detection of HAB species and investigates the relation between HAB and the specific oceanographic conditions in the region. Good agreement was obtained between microscopy and microarray data for diatoms of genus Pseudo-nitzschia and dinoflagellates Dinophysis spp., Gymnodinium catenation and raphidophyte Heterosigma akashiwo, with less effective results for Prorocentrum. Microarray provided detection of flagellates Prymnesium spp., Pseudochattonella spp., Chloromorum toxicum and the important HAB dinoflagellates of the genera Alexandrum and Azadinium, with the latter being one of the first records from the study region. Seasonality and upwelling induced by northerly winds were found to be the driving forces of HAB development, with Pseudonitzschia spp. causing the risk of ASP during spring and summer upwelling season, and dinoflagellates causing the risk of DSP and PSP during upwelling relaxation, mainly in summer and autumn. The findings were in agreement with the results from toxicity monitoring of shellfish by the Portuguese Institute for Sea and Atmosphere and confirm the suitability of the RNA microarray method for HABs detection and aquaculture management applications.
dc.description.sponsorshipEC Erasmus Mundus MACOMA PhD grant
dc.description.sponsorshipEU FP7 DEVOTES projectEuropean Union (EU) [308392]
dc.description.sponsorshipproject EU FP7 AquaUsers [607325]
dc.description.sponsorshipproject EU Horizon 2020 AquaSpace [633476]
dc.description.sponsorshipproject EU Horizon 2020 Ceres [607325]
dc.description.sponsorshipproject EU Horizon 2020 GAIN [773330]
dc.description.sponsorshipPortuguese Science Foundation (FCT)Portuguese Foundation for Science and Technology [UID/MAR/00350/2013, UID/MAR/00350/2019]
dc.identifier.doi10.1016/j.hal.2018.12.002
dc.identifier.issn1568-9883
dc.identifier.issn1878-1470
dc.identifier.urihttp://hdl.handle.net/10400.1/14416
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Science Bv
dc.subjectAzadinium-Poporum Dinophyceae
dc.subjectGenus Azadinium
dc.subjectAlgal Blooms
dc.subjectMolecular Characterization
dc.subjectAzaspiracid Profile
dc.subjectMidtal Microarray
dc.subjectAtlantic Coast
dc.subjectChlorophyll-A
dc.subjectToxic Algae
dc.subjectInner-Shelf
dc.titleHarmful phytoplankton diversity and dynamics in an upwelling region (Sagres, SW Portugal) revealed by ribosomal RNA microarray combined with microscopy
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage71
oaire.citation.startPage52
oaire.citation.titleHarmful Algae
oaire.citation.volume82
person.familyNameDanchenko
person.familyNameIcely
person.givenNameSergei
person.givenNameJohn
person.identifier.orcid0000-0002-8667-4430
person.identifier.orcid0000-0002-9114-8283
person.identifier.scopus-author-id56405638100
person.identifier.scopus-author-id6506626316
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublicatione02bf91b-5d47-4140-9108-735d89a030ef
relation.isAuthorOfPublicationd8d4a6eb-57ae-440b-99b9-5f981d237cdf
relation.isAuthorOfPublication.latestForDiscoverye02bf91b-5d47-4140-9108-735d89a030ef

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