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Moroccan propolis: a natural antioxidant, antibacterial, and antibiofilm against Staphylococcus aureus with no induction of resistance after continuous exposure

2018Journal article Open Access
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dc.contributor.authorEL-GEUNDOUZ, Soukaina
dc.contributor.authorAAZZA, Smail
dc.contributor.authorLyoussi, Badiaa
dc.contributor.authorBankova, Vassya
dc.contributor.authorPopova, Milena
dc.contributor.authorNeto, Luís
dc.contributor.authorFaleiro, Maria Leonor
dc.contributor.authorMiguel, Maria
dc.date.accessioned2019-03-07T11:57:26Z
dc.date.available2019-03-07T11:57:26Z
dc.date.issued2018
dc.description.abstractThis study was performed to evaluate the total phenols, flavonoids, and antioxidant activities of twenty-four propolis samples from different regions of Morocco. In addition, two samples were screened regarding the antibacterial effect against four Staphylococcus aureus strains. Gas chromatography coupled to mass spectra (GC-MS) analysis was done for propolis samples used in antibacterial tests. The minimum inhibitory and minimum bactericidal concentration (MIC, MBC) were determined. The potential to acquire the resistance after sequential exposure of bacterial strains and the impact of adaptation to propolis on virulence using the Galleria mellonella were evaluated. Additionally, the effects of propolis extract on the bacterial adherence ability and its ability to inhibit the quorum sensing activity were also examined. Among the twenty-four extracts studied, the samples from Sefrou, Outat el Haj, and the two samples marketed in Morocco were the best for scavenging DPPH, ABTS, NO, peroxyl, and superoxide radicals as well as in scavenging of hydrogen peroxide. A strong correlation was found between the amounts of phenols, flavonoids, and antioxidant activities. Propolis extract at the MIC value (0.36 mg/mL) significantly reduced (p < 0.001) the virulence potential of S. aureus ATCC 6538 and the MRSA strains without leading to the development of resistance in the sequence of continuous exposure. It was able to impair the bacterial biofilm formation. The results have revealed that sample 1 reduces violacein production in a concentration dependent manner, indicating inhibition of quorum sensing. This extract has as main group of secondary metabolites flavonoids (31.9%), diterpenes (21.5%), and phenolic acid esters (16.5%).pt_PT
dc.description.sponsorshipFundacao para a Ciencia e a Tecnologia (FCT) - Pest-OE/QUI/UI4023/2011pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1155/2018/9759240pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.1/12399
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherHindawi Publishing Corporationpt_PT
dc.titleMoroccan propolis: a natural antioxidant, antibacterial, and antibiofilm against Staphylococcus aureus with no induction of resistance after continuous exposurept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F00100%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F04773%2F2013/PT
oaire.citation.endPage19pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleEvidence-Based Complementary and Alternative Medicinept_PT
oaire.citation.volume2018pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameEl-Guendouz
person.familyNameAAZZA
person.familyNameNeto
person.familyNameFaleiro
person.familyNameMiguel
person.givenNameSoukaina
person.givenNameSmail
person.givenNameLuís
person.givenNameMaria Leonor
person.givenNameMaria da Graça
person.identifierhttps://scholar.google.com/citations?user=CYBI8q8AAAAJ&hl=fr
person.identifier1426445
person.identifier2096804
person.identifier252666
person.identifier.ciencia-id3E11-53A1-4D90
person.identifier.ciencia-idE416-77DF-6A97
person.identifier.ciencia-id2D16-F3CF-DDF3
person.identifier.ciencia-id281D-5E01-F09A
person.identifier.ciencia-id6110-6FE7-DF28
person.identifier.orcid0000-0002-3341-9452
person.identifier.orcid0000-0002-9339-8999
person.identifier.orcid0000-0003-1834-6459
person.identifier.orcid0000-0002-3878-6948
person.identifier.orcid0000-0003-2507-4228
person.identifier.ridL-7630-2017
person.identifier.scopus-author-id57190122389
person.identifier.scopus-author-id51664684500
person.identifier.scopus-author-id15838061200
person.identifier.scopus-author-id6507927018
person.identifier.scopus-author-id35562054500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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