Repository logo
 
Publication

Impairment of mineralization by metavanadate and decavanadate solutions in a fish bone-derived cell line

2008-06Journal article Open access
Simple item page
dc.contributor.authorTiago, Daniel
dc.contributor.authorLaizé, Vincent
dc.contributor.authorCancela, Leonor
dc.contributor.authorAureliano, M.
dc.date.accessioned2014-07-07T14:07:42Z
dc.date.available2014-07-07T14:07:42Z
dc.date.issued2008-06
dc.date.updated2014-06-03T09:50:52Z
dc.description.abstractVanadium, a trace metal known to accumulate in bone and to mimic insulin, has been shown to regulate mammalian bone formation using in vitro and in vivo systems. In the present work, short- and long-term effects of metavanadate (containing monomeric, dimeric, tetrameric and pentameric vanadate species) and decavanadate (containing decameric vanadate species) solutions on the mineralization of a fish bone-derived cell line (VSa13) were studied and compared to that of insulin. After 2 h of incubation with vanadate (10 μM in monomeric vanadate), metavanadate exhibited higher accumulation rates than decavanadate (6.85±0.40 versus 3.95±0.10 μg V/g of protein, respectively) in fish VSa13 cells and was also shown to be less toxic when applied for short periods. In longer treatments with both metavanadate and decavanadate solutions, similar effects were promoted: stimulation of cell proliferation and strong impairment (75%) of extracellular matrix (ECM) mineralization. The effect of both vanadate solutions (5 μM in monomeric vanadate), on ECM mineralization was increased in the presence of insulin (10 nM). It is concluded that chronic treatment with both vanadate solutions stimulated fish VSa13 cells proliferation and prevented ECM mineralization. Newly developed VSa13 fish cells appeared to be appropriate in the characterization of vanadate effects on vertebrate bone formation, representing a good alternative to mammalian systems.por
dc.identifier.citationTiago DM; Laizé V; Cancela ML; Aureliano M. Impairment of mineralization by metavanadate and decavanadate solutions in a fish bone-derived cell line, Cell Biology and Toxicology, 24, 3, 253-263, 2008.por
dc.identifier.doihttp://dx.doi.org/10.1007/s10565-007-9034-x
dc.identifier.otherAUT: LCA00739; MAA01296
dc.identifier.urihttp://hdl.handle.net/10400.1/4707
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relation.publisherversionhttp://link.springer.com/article/10.1007/s10565-007-9034-x/fulltext.htmlpor
dc.subjectBone-derived cell linepor
dc.subjectVanadatepor
dc.subjectInsulin-mimetic propertiespor
dc.subjectVertebrate bone formationpor
dc.subjectTeleost fish Sparus auratapor
dc.titleImpairment of mineralization by metavanadate and decavanadate solutions in a fish bone-derived cell linepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage263por
oaire.citation.issue3por
oaire.citation.startPage253por
oaire.citation.titleCell Biol Toxicolpor
oaire.citation.volume24por
person.familyNameTiago
person.familyNameLaizé
person.familyNameCancela
person.familyNameAureliano
person.givenNameDaniel
person.givenNameVincent
person.givenNameM. Leonor
person.givenNameManuel
person.identifier584146
person.identifier.ciencia-idAA14-3490-DC5E
person.identifier.orcid0000-0001-8418-6292
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0003-4858-3201
person.identifier.ridB-4463-2008
person.identifier.ridI-3283-2012
person.identifier.scopus-author-id14422711000
person.identifier.scopus-author-id6602982778
person.identifier.scopus-author-id6603412860
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication10cda3a0-7b9c-4d16-bd2a-a06df1d56d94
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublicationbb413661-7edd-4b57-8338-33889cfd05db
relation.isAuthorOfPublication.latestForDiscoveryb9bbfe32-3dfe-4131-ad14-a4394008447f

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Impairment of mineralization by metavanadate and decavanadate solutions in a fish bone-derived cell line.pdf
Size:
238.75 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)