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Tissue and salinity specific Na+/Cl− cotransporter (NCC) orthologues involved in the adaptive osmoregulation of sea lamprey (Petromyzon marinus)

2021-11Journal article Open access
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dc.contributor.authorBarany, A.
dc.contributor.authorShaughnessy, C. A.
dc.contributor.authorPelis, R. M.
dc.contributor.authorFuentes, J.
dc.contributor.authorMancera, J. M.
dc.contributor.authorMcCormick, S. D.
dc.date.accessioned2021-12-15T10:42:51Z
dc.date.available2021-12-15T10:42:51Z
dc.date.issued2021-11
dc.description.abstractTwo orthologues of the gene encoding the Na+-Cl− cotransporter (NCC), termed ncca and nccb, were found in the sea lamprey genome. No gene encoding the Na+-K+-2Cl cotransporter 2 (nkcc2) was identifed. In a phylogenetic comparison among other vertebrate NCC and NKCC sequences, the sea lamprey NCCs occupied basal positions within the NCC clades. In freshwater, ncca mRNA was found only in the gill and nccb only in the intestine, whereas both were found in the kidney. Intestinal nccb mRNA levels increased during late metamorphosis coincident with salinity tolerance. Acclimation to seawater increased nccb mRNA levels in the intestine and kidney. Electrophysiological analysis of intestinal tissue ex vivo showed this tissue was anion absorptive. After seawater acclimation, the proximal intestine became less anion absorptive, whereas the distal intestine remained unchanged. Luminal application of indapamide (an NCC inhibitor) resulted in 73% and 30% inhibition of shortcircuit current (Isc) in the proximal and distal intestine, respectively. Luminal application of bumetanide (an NKCC inhibitor) did not afect intestinal Isc. Indapamide also inhibited intestinal water absorption. Our results indicate that NCCb is likely the key ion cotransport protein for ion uptake by the lamprey intestine that facilitates water absorption in seawater. As such, the preparatory increases in intestinal nccb mRNA levels during metamorphosis of sea lamprey are likely critical to development of whole animal salinity tolerance.pt_PT
dc.description.sponsorshipPU/PP-EST/MV/2018-004
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/s41598-021-02125-1pt_PT
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.1/17387
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Researchpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSecreção de bicarbonato intestinalpt_PT
dc.subjectCotransportador NA-CLpt_PT
dc.subjectAbsorção de águapt_PT
dc.subjectExpressão genéticapt_PT
dc.subjectÁgua docept_PT
dc.subjectCaptação de íonspt_PT
dc.subjectMecanismospt_PT
dc.subjectIonócitospt_PT
dc.subjectGenomapt_PT
dc.subjectModelopt_PT
dc.titleTissue and salinity specific Na+/Cl− cotransporter (NCC) orthologues involved in the adaptive osmoregulation of sea lamprey (Petromyzon marinus)pt_PT
dc.title.alternativeOrtomologia específica de tecido e salinidade Na+/Cl-cotransporter (NCC) envolvida na osmoregulação adaptativa do lamprey marinho (Petromyzon marinus)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.citation.issue1pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameBarany
person.givenNameAndre
person.identifier.orcid0000-0001-7493-8200
person.identifier.scopus-author-id57209284558
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoverye7b3bd13-36eb-4412-b558-95afd4c385b5
relation.isProjectOfPublicationfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f
relation.isProjectOfPublication.latestForDiscoveryfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f

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