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Assessment of male reproductive traits in endangered leuciscids from the Iberian Peninsula: first attempts to store gametes both at short- and long-term

2023Journal article Open Access
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dc.contributor.authorHernandez, Ana
dc.contributor.authorSousa-Santos, Carla
dc.contributor.authorGil, Fátima
dc.contributor.authorCabrita, Elsa
dc.contributor.authorGuerreiro, Pedro
dc.contributor.authorGallego, Victor
dc.date.accessioned2023-05-30T11:21:41Z
dc.date.available2023-05-30T11:21:41Z
dc.date.issued2023
dc.description.abstractDuring the spring of 2022, several endangered leuciscid species (Anaecypris hispanica, Squalius aradensis, Anachondrostoma Occidentale, and Iberochondrostoma lusitanicum) were sampled both at the Vasco da Gama aquarium facilities and in some rivers of the Algarve region, Portugal. Sperm samples were extracted by gentle abdominal pressure and sperm motion parameters were assessed for the first time in four species, using a computerized analysis system. The results obtained showed that spermatozoa kinetic patterns were similar for all 4 species, with high motility and velocity values after the sperm activation time and with a marked decrease after 20. On the other hand, sperm longevity was highly variable between species, with short longevities (around 40 s) for A. hispanica and S. aradensis, and longer longevities (100-120 s) for A. occidentale and I. lusitanicum, which could indicate a latitudinal pattern in terms of sperm longevity. At the same time, morphometric analysis was carried out for the four target species, revealing that spermatozoa showed similar sizes and shapes to other external fertilizers belonging to Leuscididae, with small spherical heads, uniflagellate, and without acrosomes. In addition, a short-term gamete storage trail was performed by diluting sperm in 1:9 (sperm:extender) and storing them at 4oC. Although the results obtained were uneven among the species studied, the dilution and extender used generated motilities above 40% up to day 4 of storage in S. aradensis and I. lusitanicum, and up to days 1-2 in A. hispanica and A. occidentale, respectively. Finally, gamete cryopreservation trials were also carried out on these threatened species. Although cryopreserved samples showed significantly lower motility than fresh samples, some protocols generate acceptable percentages of viability, DNA integrity, and sperm motility in some species such as I. lusitanicum and A. occidentale. The data revealed that the protocol based on 10% DMSO plus 7.5% egg yolk generated the best results.This study is the first to assess the reproductive traits of wild and captive populations of endangered leuciscids endemic from the Iberian Peninsula, describing the spermatozoa kinetics and developing protocols for managing male gametes both in short- and long-term storage. Outcomes will provide new and useful tools to complement the management and conservation of ex situ breeding programs that are being developed for these four endangered species.pt_PT
dc.description.sponsorshipLA/P/0069/2020pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s10695-023-01195-4pt_PT
dc.identifier.issn0920-1742
dc.identifier.urihttp://hdl.handle.net/10400.1/19638
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSPRINGERpt_PT
dc.relationMarine and Environmental Sciences Centre
dc.relationMarine and Environmental Sciences Centre
dc.relationGamete and embryo cryobanking for the preservation of threatened endemic fish
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectLeuciscidaept_PT
dc.subjectSperm motilitypt_PT
dc.subjectShort-term storagept_PT
dc.subjectSperm cryopreservationpt_PT
dc.titleAssessment of male reproductive traits in endangered leuciscids from the Iberian Peninsula: first attempts to store gametes both at short- and long-termpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleGamete and embryo cryobanking for the preservation of threatened endemic fish
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/101038049/EU
oaire.citation.titleFish Physiology and Biochemistrypt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamH2020
person.familyNameHernandez
person.familyNameCabrita
person.familyNameGuerreiro da Costa Guerreiro
person.familyNameGallego
person.givenNameAna
person.givenNameElsa
person.givenNamePedro Miguel
person.givenNameVictor
person.identifierA-2539-2009
person.identifier.ciencia-id5C13-965D-3148
person.identifier.ciencia-id8B13-2B8A-EDDE
person.identifier.orcid0000-0002-0421-0318
person.identifier.orcid0000-0003-3864-8841
person.identifier.orcid0000-0001-5371-7919
person.identifier.orcid0000-0002-9733-0625
person.identifier.ridE-8523-2012
person.identifier.ridK-9072-2017
person.identifier.scopus-author-id26645697000
person.identifier.scopus-author-id33367852200
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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