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New insights into benzo[⍺]pyrene osteotoxicity in zebrafish

2021-12Journal article Open access
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dc.contributor.authorTarasco, Marco
dc.contributor.authorGavaia, Paulo
dc.contributor.authorBensimon-Brito, Anabela
dc.contributor.authorCardeira-da-Silva, João
dc.contributor.authorRamkumar, Srinath
dc.contributor.authorCordelières, Fabrice P.
dc.contributor.authorGünther, Stefan
dc.contributor.authorBebianno, Maria J.
dc.contributor.authorStainier, Didier Y.R.
dc.contributor.authorCancela, M. Leonor
dc.contributor.authorLaizé, Vincent
dc.date.accessioned2021-11-06T12:45:03Z
dc.date.available2021-11-06T12:45:03Z
dc.date.issued2021-12
dc.description.abstractPersistent and ubiquitous organic pollutants, such as the polycyclic aromatic hydrocarbon benzo[⍺]pyrene (BaP), represent a major threat to aquatic organisms and human health. Beside some well-documented adverse effects on the development and reproduction of aquatic organisms, BaP was recently shown to affect fish bone formation and skeletal development through mechanisms that remain poorly understood. In this work, zebrafish bonerelated in vivo assays were used to evaluate the osteotoxic effects of BaP during bone development and regeneration. Acute exposure of zebrafish larvae to BaP from 3 to 6 days post-fertilization (dpf) induced a dosedependent reduction of the opercular bone size and a depletion of osteocalcin-positive cells, indicating an effect on osteoblast maturation. Chronic exposure of zebrafish larvae to BaP from 3 to 30 dpf affected the development of the axial skeleton and increased the incidence and severity of skeletal deformities. In young adults, BaP affected the mineralization of newly formed fin rays and scales, and impaired fin ray patterning and scale shape, through mechanisms that involve an imbalanced bone remodeling. Gene expression analyses indicated that BaP induced the activation of xenobiotic and metabolic pathways, while negatively impacting extracellular matrix formation and organization. Interestingly, BaP exposure positively regulated inflammation markers in larvae and increased the recruitment of neutrophils. A direct interaction between neutrophils and bone extracellular matrix or bone forming cells was observed in vivo, suggesting a role for neutrophils in the mechanisms underlying BaP osteotoxicity. Our work provides novel data on the cellular and molecular players involved in BaP osteotoxicity and brings new insights into a possible role for neutrophils in inflammatory bone reduction.pt_PT
dc.description.sponsorshipMAR-02.01.01-FEAMP-0057; CA15124; ANR-10-INBS-04
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.ecoenv.2021.112838pt_PT
dc.identifier.eissn1090-2414
dc.identifier.urihttp://hdl.handle.net/10400.1/17298
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAcademic Press Inc Elsevier Sciencept_PT
dc.relationHow microplastics and adsorbed pollutants affect zebrafish development
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectOpercular bone growthpt_PT
dc.subjectBone matrixpt_PT
dc.subjectCaudal fin and scale regenerationpt_PT
dc.subjectXenobiotic signalingpt_PT
dc.subjectNeutrophilspt_PT
dc.titleNew insights into benzo[⍺]pyrene osteotoxicity in zebrafishpt_PT
dc.title.alternativeNovos insights sobre a osteotoxicidade do benzo[alfa]pirinete em zebrafishpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHow microplastics and adsorbed pollutants affect zebrafish development
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F128634%2F2017/PT
oaire.citation.startPage112838pt_PT
oaire.citation.titleEcotoxicology and Environmental Safetypt_PT
oaire.citation.volume226pt_PT
oaire.fundingStreamOE
person.familyNameTarasco
person.familyNameGavaia
person.familyNameCancela
person.familyNameLaizé
person.givenNameMarco
person.givenNamePaulo
person.givenNameM. Leonor
person.givenNameVincent
person.identifier.ciencia-id2B1F-0392-9ABE
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0001-5894-6526
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0001-9565-9198
person.identifier.ridG-9575-2017
person.identifier.ridA-6470-2011
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id57194105105
person.identifier.scopus-author-id6507104377
person.identifier.scopus-author-id6602982778
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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