dc.contributor.author	Vieira, Florbela A.
dc.contributor.author	Gregorio, Silvia
dc.contributor.author	Ferraresso, Serena
dc.contributor.author	Thorne, Michael A. S.
dc.contributor.author	Costa, Rita
dc.contributor.author	Milan, Massimo
dc.contributor.author	Bargelloni, Luca
dc.contributor.author	Clark, M. S.
dc.contributor.author	Canario, Adelino V. M.
dc.contributor.author	Power, Deborah
dc.date.accessioned	2011-10-26T21:57:47Z
dc.date.available	2011-10-26T21:57:47Z
dc.date.issued	2011-10-07
dc.date.updated	2011-10-23T19:07:35Z
dc.description.abstract	Abstract Background Fish scales are an important reservoir of calcium and phosphorus and together with the skin function as an integrated barrier against environmental changes and external aggressors. Histological studies have revealed that the skin and scales regenerate rapidly in fish when they are lost or damaged. In the present manuscript the histological and molecular changes underlying skin and scale regeneration in fed and fasted sea bream (Sparus auratus) were studied using a microarray 3 and 7 days after scale removal to provide a comprehensive molecular understanding of the early stages of these processes. Results Histological analysis of skin/scales revealed 3 days after scale removal re-epithelisation and formation of the scale pocket had occurred and 53 and 109 genes showed significant up or down-regulation, respectively. Genes significantly up-regulated were involved in cell cycle regulation, cell proliferation and adhesion, immune response and antioxidant activities. 7 days after scale removal a thin regenerated scale was visible and only minor changes in gene expression occurred. In animals that were fasted to deplete mineral availability the expression profiles centred on maintaining energy homeostasis. The utilisation of fasting as a treatment emphasised the competing whole animal physiological requirements with regard to barrier repair, infection control and energy homeostasis. Conclusions The identification of numerous genes involved in the mitotic checkpoint and cell proliferation indicate that the experimental procedure may be useful for understanding cell proliferation and control in vertebrates within the context of the whole animal physiology. In response to skin damage genes of immune surveillance were up-regulated along with others involved in tissue regeneration required to rapidly re-establish barrier function. Additionally, candidate fish genes were identified that may be involved in cytoskeletal re-modelling, mineralization and stem cells, which are of potential use in aquaculture and fish husbandry, as they may impact on the ability of the fish to produce structural proteins, such as muscle, efficiently.	por
dc.description.version	info:eu-repo/semantics/publishedVersion
dc.identifier.citation	BMC Genomics. 2011 Oct 07;12(1):490	por
dc.identifier.doi	http://dx.doi.org/10.1186/1471-2164-12-490
dc.identifier.other	AUT: ACA00258; DPO00386;
dc.identifier.uri	http://hdl.handle.net/10400.1/866
dc.language.iso	eng	por
dc.language.rfc3066	eng
dc.peerreviewed	yes	por
dc.rights.holder	open access
dc.title	Skin healing and scale regeneration in fed and unfed sea bream, Sparus auratus	por
dc.type	journal article
dspace.entity.type	Publication
person.familyName	Gregorio
person.familyName	Canario
person.familyName	Power
person.givenName	Silvia
person.givenName	Adelino
person.givenName	Deborah Mary
person.identifier	1579837
person.identifier	143624
person.identifier.ciencia-id	BF14-020B-4C39
person.identifier.ciencia-id	1F1E-D3B3-F804
person.identifier.ciencia-id	891A-8A44-3CAE
person.identifier.orcid	0000-0002-6648-5122
person.identifier.orcid	0000-0002-6244-6468
person.identifier.orcid	0000-0003-1366-0246
person.identifier.rid	G-1618-2018
person.identifier.rid	C-7942-2009
person.identifier.scopus-author-id	56568523700
person.identifier.scopus-author-id	7101806760
rcaap.rights	openAccess	por
rcaap.type	article	por
relation.isAuthorOfPublication	9bc940d8-7dfb-4950-98f4-6853f398cb49
relation.isAuthorOfPublication	5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication	c68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery	9bc940d8-7dfb-4950-98f4-6853f398cb49

Skin healing and scale regeneration in fed and unfed sea bream, Sparus auratus

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