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Ubiquitination and carbonylation as markers of oxidative-stress in Ruditapes decussatus

2008Journal article Restricted access
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dc.contributor.authorChora, Suze
dc.contributor.authorMcDonagh, Brian
dc.contributor.authorSheehan, David
dc.contributor.authorStarita-Geribaldi, Mireille
dc.contributor.authorRoméo, Michèle
dc.contributor.authorBebianno, Maria João
dc.date.accessioned2020-05-05T08:19:51Z
dc.date.available2020-05-05T08:19:51Z
dc.date.issued2008
dc.description.abstractEnvironmental pollutants, such as metals, are widespread in aquatic environments and can lead to the formation of reactive oxygen species (ROS). ROS are highly toxic in marine species since they can cause serious reversible and irreversible changes in proteins including ubiquitination and modifications such as carbonylation. This study aimed to confirm the potential of ubiquitination and carbonylation as markers of oxidative stress in the clam Ruditapes decussatus (Veneroida, Veneridae) exposed to cadmium (40 microg/L). After 21 days of exposure clams were dissected into gills and digestive gland. Cytosolic proteins of both tissues were separated by two-dimensional electrophoresis (2-D SDS-PAGE) and analysed by immunobloting. Higher ubiquitination and carbonylation levels were in digestive gland of contaminated organisms. These results confirm the potential of ubiquitination and carbonylation as a sensitive and specific marker of oxidative stress in marine bivalves. In this approach, changes in protein structure provide options for affinity selection of sub-proteomes for 2D SDS-PAGE, simplifying the detection of protein biomarkers using proteomic approach.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.marenvres.2008.02.034pt_PT
dc.identifier.issn0141-1136
dc.identifier.urihttp://hdl.handle.net/10400.1/13828
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationESTUDO DO EFEITO DE CONTAMINANTES QUÍMICOS NUMA ESPÉCIE SENTINELA, A AMÊIJOA RUDITAPES DECUSSATUS ATRAVÉS DA ANÁLISE DA MODIFICAÇÃO DA EXPRESSÃO PROTEICA E GENÓMICA: APLICAÇÃO À MONITORIZAÇÃO DO AMBIENTE MARINHO
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectBiomarkerspt_PT
dc.subjectBivalviapt_PT
dc.subjectCadmiumpt_PT
dc.subjectDigestive systempt_PT
dc.subjectGillspt_PT
dc.subjectOxidative stresspt_PT
dc.subjectProtein carbonylationpt_PT
dc.subjectUbiquitinationpt_PT
dc.subjectWater pollutantspt_PT
dc.subjectChemicalpt_PT
dc.titleUbiquitination and carbonylation as markers of oxidative-stress in Ruditapes decussatuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleESTUDO DO EFEITO DE CONTAMINANTES QUÍMICOS NUMA ESPÉCIE SENTINELA, A AMÊIJOA RUDITAPES DECUSSATUS ATRAVÉS DA ANÁLISE DA MODIFICAÇÃO DA EXPRESSÃO PROTEICA E GENÓMICA: APLICAÇÃO À MONITORIZAÇÃO DO AMBIENTE MARINHO
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F19477%2F2004/PT
oaire.citation.endPage97pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage95pt_PT
oaire.citation.titleMarine Environmental Researchpt_PT
oaire.citation.volume66pt_PT
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isProjectOfPublicationa7f5f67d-82b6-475d-ba23-0955ed06e185
relation.isProjectOfPublication.latestForDiscoverya7f5f67d-82b6-475d-ba23-0955ed06e185

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