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Effect of cadmium in the clam Ruditapes decussatus assessed by proteomic analysis

2009Journal article Restricted access
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dc.contributor.authorChora, Suze
dc.contributor.authorStarita-Geribaldi, Mireille
dc.contributor.authorGuigonis, Jean-Marie
dc.contributor.authorSamson, Michel
dc.contributor.authorRoméo, Michèle
dc.contributor.authorBebianno, Maria João
dc.date.accessioned2020-04-29T12:30:42Z
dc.date.available2020-04-29T12:30:42Z
dc.date.issued2009
dc.description.abstractCadmium, an environmental stressor due to its toxicity, persistence and accumulation in biota, is widespread in the aquatic environment. Cadmium accumulation kinetics have revealed that Ruditapes decussatus has a high affinity to this metal. Proteomics is an effective tool to evaluate the toxic effects of contaminants. The aim of this study was to investigate the Cd effects in the gill and digestive gland of the sentinel species R. decussatus. Protein expression profiles (PEPs) in the clam tissues exposed to Cd (40 microg l(-1), 21 days) were compared to unexposed ones. Cd induces major changes in tissue-specific protein expression profiles in gill and digestive gland. This tissue dependent response results mainly from differences in Cd accumulation, protein inhibition and/or autophagy. An overall decrease of protein spots was detected in both treated tissues, being higher in gill. Some of the spots more drastically altered after pollutants exposure were excised and nine were identified by micro liquid chromatography tandem mass spectrometry (LC-MS/MS). Proteins identified by homology search in databases included: three proteins (8-fold) up-regulated, one down-regulated, four suppressed and one induced. Cd induces major changes in proteins involved in cytoskeletal structure maintenance (muscle-type actin, adductor muscle actin and beta-tubulin), cell maintenance (Rab GDP) and metabolism (ALDH and MCAD, both identified by de novo sequencing) suggesting potential energetic change. They provide a valuable knowledge of Cd effects at biochemical and molecular levels in the gill and digestive gland of R. decussatus.pt_PT
dc.description.sponsorshipPortuguese Foundation for Science and Technologypt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttps://doi.org/10.1016/j.aquatox.2009.07.014pt_PT
dc.identifier.issn0166-445X
dc.identifier.urihttp://hdl.handle.net/10400.1/13802
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0166445X09002501?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectBivalviapt_PT
dc.subjectCadmiumpt_PT
dc.subjectChromatographypt_PT
dc.subjectDigestive systempt_PT
dc.subjectGillspt_PT
dc.subjectProteomept_PT
dc.subjectProteomicspt_PT
dc.subjectTandem mass spectrometrypt_PT
dc.subjectTissue distributionpt_PT
dc.subjectProteomicpt_PT
dc.subjectRuditapes decussatuspt_PT
dc.subjectLC-MS/MSpt_PT
dc.titleEffect of cadmium in the clam Ruditapes decussatus assessed by proteomic analysispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage308pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage300pt_PT
oaire.citation.titleAquatic Toxicologypt_PT
oaire.citation.volume94pt_PT
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32

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