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The protective effect of endogenous melatonin on gilthead seabream sperm during cryopreservation

2023Journal article Open access
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dc.contributor.authorFélix, Francisca
dc.contributor.authorAntunes, Raquel
dc.contributor.authorVera, L. M.
dc.contributor.authorOliveira, Catarina
dc.contributor.authorCabrita, Elsa
dc.date.accessioned2023-11-07T15:33:42Z
dc.date.available2023-11-07T15:33:42Z
dc.date.issued2023
dc.description.abstractCryopreservation is widely used for artificial reproduction techniques (ART) but requires good gamete quality to succeed. Considering the endogenously produced melatonin by the fish and its protective role in the organism, the objective of this experiment was to search for the best moment of the day to collect gilthead seabream (Sparus aurata) sperm with better quality and, at the same time, to test the potential protective effect of the exogenous melatonin during sperm freezing and thawing processes. Fish were first sampled every 6 h, corresponding to the beginning of the light period (6 h), mid-light (12h), beginning of the dark period (18 h), and mid-dark (24 h) to determine melatonin concentration in blood plasma by radioimmunoassay (RIA). The analysis showed higher values at 24 h (244 pg/mL) compared with 12 h (99 pg/mL), which allowed the selection of those moments for the next experiment. After, fish were sampled for sperm collection at mid-light (ML, 12 h) and mid-dark (MD, 24 h), and fresh sperm was used to assess gamete quality and for the cryopreservation assay. Sperm samples were cryopreserved to test supplementation of different melatonin concentrations (0.001 mM, 0.01 mM and 0.1 mM) together with a control group without added melatonin. Gamete quality was assessed through spermatozoa concentration and motility (CASA system), cell viability (PI/SYBR-green) and DNA fragmentation (Comet assay). Despite cell viability that was higher at ML, most of the fresh sperm motility parameters did not differ between ML and MD, only linearity (LIN) was enhanced at MD. Nevertheless, in cryopreserved samples, total motility (TM) was significantly higher at MD in all melatonin treatments, control, and fresh samples, revealing an endogenous night-effect. Moreover, spermatozoa concentration was also higher at MD (28.9 x 109/mL) than at ML (20.7 x 109/mL). Supplemented melatonin did not confer extra protection to gilthead seabream sperm during cryopreservation since the tested concentrations did not differ between the control in any sperm quality test. It is here suggested that endogenously produced melatonin may contribute to the improvement of some gamete quality parameters at mid-dark, allowing the aquaculture sector to select better sperm quality in a noninvasive way by choosing it as the best moment of the day for sperm collection.pt_PT
dc.description.sponsorshipLA/P/0101/2020pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.aquaculture.2023.739997pt_PT
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10400.1/20138
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationImprovement of Senegalese sole sperm quality through the action of melatonin as an antioxidant
dc.relationNot Available
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSparus auratapt_PT
dc.subjectSpermatozoapt_PT
dc.subjectExtenderpt_PT
dc.subjectSupplementationpt_PT
dc.subjectAntioxidantspt_PT
dc.titleThe protective effect of endogenous melatonin on gilthead seabream sperm during cryopreservationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleImprovement of Senegalese sole sperm quality through the action of melatonin as an antioxidant
oaire.awardTitleNot Available
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F148280%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL 57%2F2016%2FCP1361%2FCT0007/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.citation.startPage739997pt_PT
oaire.citation.titleAquaculturept_PT
oaire.citation.volume577pt_PT
oaire.fundingStreamDL 57/2016
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFélix de Azeredo Pinto e Melo
person.familyNameAntunes
person.familyNameOliveira
person.familyNameCabrita
person.givenNameFrancisca
person.givenNameRaquel
person.givenNameCatarina
person.givenNameElsa
person.identifier.ciencia-idAC1C-FB89-E69C
person.identifier.ciencia-id0816-30C5-51AC
person.identifier.orcid0009-0002-8391-3165
person.identifier.orcid0000-0002-6679-761X
person.identifier.orcid0000-0003-3864-8841
person.identifier.ridE-8523-2012
person.identifier.scopus-author-id58548266900
person.identifier.scopus-author-id35433190500
person.identifier.scopus-author-id26645697000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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