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RNAi-mediated knock-down of the dopamine beta-hydroxylase gene changes growth of razor clams

2021-03Journal article Restricted access
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dc.contributor.authorLi, Zhi
dc.contributor.authorPeng, Maoxiao
dc.contributor.authorPower, Deborah
dc.contributor.authorNiu, Donghong
dc.contributor.authorDong, Zhiguo
dc.contributor.authorLi, Jiale
dc.date.accessioned2021-09-08T10:58:09Z
dc.date.available2021-09-08T10:58:09Z
dc.date.issued2021-03
dc.description.abstractDopamine beta-hydroxylase (D beta H) plays an essential role in the synthesis of catecholamines (CA) in neuroen-docrine networks. In the razor clam, Sinonovacula constricta a novel gene for D beta H (ScD beta H-alpha) was identified that belongs to the copper type II ascorbate-dependent monooxygenase family. Expression analysis revealed ScD beta H-alpha gene transcripts were abundant in the liver and expressed throughout development. Knock-down of ScD beta H-alpha in adult clams using siRNA caused a reduction in the growth rate compared to control clams. Reduced growth was associated with strong down-regulation of gene transcripts for the growth-related factors, platelet derived growth factors A (PDGF-A) (P < 0.001) 24 h after ScD beta H-alpha knock-down, vascular endothelial growth factor (VEGF1) (P < 0.001) and platelet derived growth factor B (PDGF-B-2)(P < 0.001) 24 h and 48 h after ScD beta H-alpha knock-down and transforming growth factor beta (TGF-beta 1) (P < 0.001) 48 h and 72 h after ScD beta H-alpha knock-down. Taken together the results suggest that the novel ScD beta H-alpha gene through its role in CA synthesis is involved in growth regulation in the razor clam and possibly other bivalves.
dc.description.sponsorshipNational Key R & D Plan "Blue Granary Science and Technology Innovation" special project [2019YFD0900400]; Jiangsu Key Laboratory of Marine Biotechnology [HS2019002]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31472278]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.cbpb.2020.110534
dc.identifier.issn1096-4959
dc.identifier.urihttp://hdl.handle.net/10400.1/17043
dc.language.isoeng
dc.peerreviewedyes
dc.publisherELSEVIER SCIENCE INC
dc.subjectCatecholamine
dc.subjectDopamine beta-hydroxylase
dc.subjectGrowth
dc.subjectsiRNA
dc.subjectSinonovacula constricta
dc.subject.otherBiochemistry & Molecular Biology; Zoology
dc.titleRNAi-mediated knock-down of the dopamine beta-hydroxylase gene changes growth of razor clams
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage110534
oaire.citation.titleComparative Biochemistry and Physiology B-Biochemistry & Molecular Biology
oaire.citation.volume252
person.familyNamePeng
person.familyNamePower
person.givenNameMaoxiao
person.givenNameDeborah Mary
person.identifier.ciencia-id0A1D-96B9-27EB
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-4855-1202
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication43d269d9-e043-4d27-b3ee-5f1f657dcd37
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery43d269d9-e043-4d27-b3ee-5f1f657dcd37

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