Repository logo
 
Publication

Immunochemical detection of parathyroid hormone-related protein in the saccus vasculosus of a teleost fish

1996-01Journal article Restricted access
Simple item page
dc.contributor.authorDevlin, AJ
dc.contributor.authorDanks, JA
dc.contributor.authorFaulkner, MK
dc.contributor.authorPower, DM
dc.contributor.authorCanario, AVM
dc.contributor.authorMartin, TJ
dc.contributor.authorIngleton, P. M.
dc.date.accessioned2018-12-07T14:53:12Z
dc.date.available2018-12-07T14:53:12Z
dc.date.issued1996-01
dc.description.abstractUsing antisera to regions of human parathyroid hormone-related protein (PTHrP) the saccus vasculosus (SV) of the sea bream (Sparus aurata) has been shown to contain immunoreactive PTHrP. By immunohistochemistry (IHC) the epithelial coronet cells in fixed and wax-embedded SV tissue reacted with antisera to the prepro region of human PTHrP (-13 to +2), the N-terminus PTHrP (1-16), and the midmolecule PTHrP (50-69). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of saccus extracts and incubation media contained two major proteins of 14.3 and 15 kDa. By Western blotting these two proteins both reacted with the three antisera used for IHC, suggesting that they are immunochemically similar to human PTHrP (1-84). Ultrastructurally the coronet cells of Sparus saccus vasculosus resembled coronet cells described for other teleosts, with an abundant smooth endoplasmic reticulum (SER) which was more highly organized in the coronets. IHC at EM level showed reaction mainly with the membranes of the SER. These results suggest that S. aurata saccus vasculosus may produce a PTHrP-like molecule similar to human PTHrP. (C) 1996 Academic Press, Inc.
dc.description.sponsorshipWellcome Trust
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1006/gcen.1996.0010
dc.identifier.issn0016-6480
dc.identifier.urihttp://hdl.handle.net/10400.1/11398
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAcademic Press Inc Jnl-Comp Subscriptions
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSalmo-Gairdneri Richardson
dc.subjectImmunohistochemical localization
dc.subjectRainbow-trout
dc.subjectCalcium
dc.subjectCells
dc.titleImmunochemical detection of parathyroid hormone-related protein in the saccus vasculosus of a teleost fish
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage90
oaire.citation.issue1
oaire.citation.startPage83
oaire.citation.titleGeneral and Comparative Endocrinology
oaire.citation.volume101
person.familyNamePower
person.familyNameCanario
person.givenNameDeborah Mary
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
handle11398.pdf
Size:
1.4 MB
Format:
Adobe Portable Document Format
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)