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The influence of 17β-oestradiol on lymphopoiesis and immune system ontogenesis in juvenile sea bass, Dicentrarchus labrax

2021-05Journal article Restricted access
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dc.contributor.authorMoreira, Catarina
dc.contributor.authorPaiola, Matthieu
dc.contributor.authorDuflot, Aurélie
dc.contributor.authorVaró, Inma
dc.contributor.authorSitjà-Bobadilla, Ariadna
dc.contributor.authorKnigge, Thomas
dc.contributor.authorPinto, P
dc.contributor.authorMonsinjon, Tiphaine
dc.date.accessioned2022-01-17T15:39:03Z
dc.date.available2022-01-17T15:39:03Z
dc.date.issued2021-05
dc.description.abstractThe female sex steroid 17β-oestradiol (E2) is involved in the regulation of numerous physiological functions, including the immune system development and performance. The role of oestrogens during ontogenesis is, however, not well studied. In rodents and fish, thymus maturation appears to be oestrogen-dependent. Never theless, little is known about the function of oestrogen in immune system development. To further the under standing of the role of oestrogens in fish immune system ontogenesis, fingerlings of European sea bass (Dicentrarchus labrax) were exposed for 30 days to 20 ng E2⋅L− 1 , at two ages tightly related to thymic maturation, i.e., 60 or 90 days post hatch (dph). The expression of nuclear and membrane oestrogen receptors was measured in the thymus and spleen, and the expression of several T cell-related gene markers was studied in both immune organs, as well as in the liver. Waterborne E2-exposure at 20.2 ± 2.1 (S.E.) ng⋅L− 1 was confirmed by radioimmunoassay, leading to significantly higher E2-contents in the liver of exposed fish. The majority of gene markers presented age-dependent dynamics in at least one of the organs, confirming thymus maturation, but also suggesting a critical ontogenetic window for the implementation of liver resident γδ and αβ T cells. The oestrogen receptors, however, remained unchanged over the age and treatment comparisons with the exception of esr2b, which was modulated by E2 in the younger cohort and increased its expression with age in the thymus of the older cohort, as did the membrane oestrogen receptor gpera. These results confirm that oestrogen-signalling is involved in thymus maturation in European sea bass, as it is in mammals. This suggests that esr2b and gpera play key roles during thymus ontogenesis, particularly during medulla maturation. In contrast, the spleen expressed low or non-detectable levels of oestrogen receptors. The E2-exposure decreased the expression of tcrγ in the liver in the cohort exposed from 93 to 122 dph, but not the expression of any other immune-related gene analysed. These results indicate that the proliferation/migration of these innate-like T cell populations is estrogensensitive. In regard to the apparent prominent role of oestrogen-signalling in the late thymus maturation stage, the thymic differentiation of the corresponding subpopulations of T cells might be regulated by oestrogen. To the best of our knowledge, this is the first study investigating the dynamics of both nuclear and membrane oestrogen receptors in specific immune organs in a teleost fish at very early stages of immune system development as well as to examine thymic function in sea bass after an exposure to E2 during ontogenesis.pt_PT
dc.description.sponsorshipSR02022018976f; DL 57/2016/CP6321/CT0015; AE050029
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.dci.2021.104011pt_PT
dc.identifier.eissn1879-0089
dc.identifier.urihttp://hdl.handle.net/10400.1/17488
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAQUAculture infrastructures for EXCELlence in European fish research towards 2020
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectLiverpt_PT
dc.subjectOestrogen receptorpt_PT
dc.subjectT cellpt_PT
dc.subjectTeleost fishpt_PT
dc.subjectThymuspt_PT
dc.subjectSpleenpt_PT
dc.titleThe influence of 17β-oestradiol on lymphopoiesis and immune system ontogenesis in juvenile sea bass, Dicentrarchus labraxpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAQUAculture infrastructures for EXCELlence in European fish research towards 2020
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F84033%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/652831/EU
oaire.citation.startPage104011pt_PT
oaire.citation.titleDevelopmental & Comparative Immunologypt_PT
oaire.citation.volume118pt_PT
oaire.fundingStreamSFRH
oaire.fundingStreamH2020
person.familyNamePinto
person.givenNamePatricia IS
person.identifier643457
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.orcid0000-0001-7854-3898
person.identifier.ridM-3817-2013
person.identifier.scopus-author-id10240774300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication64bc526e-5281-44e5-91eb-5743436109f9
relation.isAuthorOfPublication.latestForDiscovery64bc526e-5281-44e5-91eb-5743436109f9
relation.isProjectOfPublication0f602f0c-00f1-4309-953a-58ef0de1ee6f
relation.isProjectOfPublicationaf2e9e71-3f77-4149-8f42-1d186fc443e4
relation.isProjectOfPublication.latestForDiscoveryaf2e9e71-3f77-4149-8f42-1d186fc443e4

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