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Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development

2010Journal article Open access
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dc.contributor.authorSheeba, Caroline J.
dc.contributor.authorAndrade, Raquel P.
dc.contributor.authorDuprez, Delphine
dc.contributor.authorPalmeirim, Isabel
dc.date.accessioned2018-12-07T14:58:33Z
dc.date.available2018-12-07T14:58:33Z
dc.date.issued2010
dc.description.abstractSpecific interactions between fibroblast growth factors (Fgf1-22) and their tyrosine kinase receptors (FgfR1-4) activate different signalling pathways that are responsible for the biological processes in which Fgf signalling is implicated during embryonic development. In the chick, several Fgf ligands (Fgf2, 4,8, 9, 10, 12, 13 and 18) and the four FgfRs (FgfR 1, 2, 3 and 4) have been reported to be expressed in the developing limb. The precise spatial and temporal expression of these transcripts is important to guide the limb bud to develop into a wing/leg. In this paper, we present a detailed and systematic analysis of the expression patterns of FgfR1, 2, 3 and 4 throughout chick wing development, by in situ hybridisation on whole mounts and sections. Moreover, we characterize for the first time the different isoforms of FGFR1-3 by analysing their differential expression in limb ectoderm and mesodermal tissues, using RT-PCR and in situ hybridisation on sections. Finally, isoform-specific sequences for FgfR1IIIb, FgfR1IIIc, FgfR3IIIb and FgfR3IIIc were determined and deposited in GenBank with the following accession numbers: GU053725, GU065444, GU053726, GU065445, respectively.
dc.description.sponsorshipFundacao para a Ciencia e Tecnologia [SFRH/BD/33176/2007]; Portuguese Government; Fundacao para a Ciencia e Tecnologia, Portugal [OBD/099758/2008]; EU; IBB/CBME
dc.description.sponsorshipEU/FP6 - Network of Excellence
dc.description.sponsorshipIBB/CBME, LA, FEDER/POCI 2010
dc.identifier.doi10.1387/ijdb.092887cs
dc.identifier.issn0214-6282
dc.identifier.urihttp://hdl.handle.net/10400.1/12086
dc.language.isoeng
dc.peerreviewedyes
dc.publisherUBC Press
dc.relationESTUDO DE INTERACÇÕES ENTRE O RELÓGIO MOLECULAR E A WAVEFRONT ASSOCIADOS À SEGMENTAÇÃO DE EMBRIÕES DE VERTEBRADOS
dc.relationMolecular parallelisms in vertebrate somitogenesis and limb development
dc.subjectApical ectodermal ridge
dc.subjectChondrogenesis In-Vitro
dc.subjectLimb development
dc.subjectMessenger-Rna
dc.subjectConditional Inactivation
dc.subjectSkeletal-Muscle
dc.subjectVertebrate limb
dc.subjectFgf receptor-2
dc.subjectFactor family
dc.subjectBone-growth
dc.titleComprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleESTUDO DE INTERACÇÕES ENTRE O RELÓGIO MOLECULAR E A WAVEFRONT ASSOCIADOS À SEGMENTAÇÃO DE EMBRIÕES DE VERTEBRADOS
oaire.awardTitleMolecular parallelisms in vertebrate somitogenesis and limb development
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F33176%2F2007/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-OBD%2F099758%2F2008/PT
oaire.citation.endPage1526
oaire.citation.issue10
oaire.citation.startPage1517
oaire.citation.titleInternational Journal of Developmental Biology
oaire.citation.volume54
oaire.fundingStream3599-PPCDT
person.familyNameAndrade
person.familyNamePalmeirim
person.givenNameRaquel
person.givenNameIsabel
person.identifier.ciencia-id2312-360B-227A
person.identifier.ciencia-id141A-4DE2-6FF3
person.identifier.orcid0000-0002-0397-5917
person.identifier.orcid0000-0003-2282-7829
person.identifier.scopus-author-id7103114918
person.identifier.scopus-author-id6603305227
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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