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Dysregulation of intestinal physiology by Aflatoxicosis in the Gilthead Seabream (Sparus aurata)

2021-12-20Journal article Open access
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dc.contributor.authorBarany, Andre
dc.contributor.authorOliva, Milagrosa
dc.contributor.authorGregorio, Silvia
dc.contributor.authorMartínez-Rodríguez, Gonzalo
dc.contributor.authorMancera, Juan Miguel
dc.contributor.authorFuentes, Juan
dc.date.accessioned2022-01-17T14:47:37Z
dc.date.available2022-01-17T14:47:37Z
dc.date.issued2021-12-20
dc.description.abstractAflatoxin B1 (AFB1) is a mycotoxin often present in food. This study aimed to understand the physiological effects of AFB1 on the seabream (Sparus aurata) gastrointestinal system. In a first in vitro approach, we investigated ion transport using the short-circuit current (Isc) technique in Ussing chambers in the anterior intestine (AI). Application of apical/ luminal AFB1 concentrations of 8 and 16μM to healthy tissues was without effect on tissue transepithelial electrical resistance (TER), and apparent tissue permeability (Papp) was measured using fluorescein FITC (4 kD). However, it resulted in dose-related effects on Isc. In a second approach, seabream juveniles fed with different AFB1 concentrations (1 and 2mg AFB1 kg−1 fish feed) for 85days showed significantly reduced gill Na+ /K+-ATPase (NKA) and H+-ATPase (HA) activities in the posterior intestine (PI). Moreover, dietary AFB1 modified Isc in the AI and PI, significantly affecting TER in the AI. To understand this effect on TER, we analyzed the expression of nine claudins and three occludins as markers of intestinal architecture and permeability using qPCR. Around 80% of the genes presented significantly different relative mRNA expression between AI and PI and had concomitant sensitivity to dietary AFB1. Based on the results of our in vitro, in vivo, and molecular approaches, we conclude that the effects of dietary AFB1 in the gastrointestinal system are at the base of the previously reported growth impairment caused by AFB1 in fish.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3389/fphys.2021.741192pt_PT
dc.identifier.eissn1664-042X
dc.identifier.urihttp://hdl.handle.net/10400.1/17487
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Media SApt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEpithelial barrierpt_PT
dc.subjectGastrointestinal tractpt_PT
dc.subjectMycotoxinpt_PT
dc.subjectTight junctionspt_PT
dc.subjectUssing chamberpt_PT
dc.titleDysregulation of intestinal physiology by Aflatoxicosis in the Gilthead Seabream (Sparus aurata)pt_PT
dc.title.alternativeDistria da fisiologia intestinal por Aflatoxicosis no Gilthead Seabream (Sparus aurata)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.citation.titleFrontiers in Physiologypt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGregorio
person.familyNameFuentes
person.givenNameSilvia
person.givenNameJuan
person.identifier1579837
person.identifier392998
person.identifier.ciencia-idBF14-020B-4C39
person.identifier.ciencia-id421B-E196-2C33
person.identifier.orcid0000-0002-6648-5122
person.identifier.orcid0000-0003-0430-8734
person.identifier.ridG-1618-2018
person.identifier.scopus-author-id7201832526
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9bc940d8-7dfb-4950-98f4-6853f398cb49
relation.isAuthorOfPublicationd354b937-b18c-4ca5-a843-04a04269d60f
relation.isAuthorOfPublication.latestForDiscovery9bc940d8-7dfb-4950-98f4-6853f398cb49
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relation.isProjectOfPublication.latestForDiscoveryfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f

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