Publication
Enhancing cryopreservation of human iPSCs: Bottom-up vs Conventional freezing geometry
| dc.contributor.author | Morais, Fernando Jorge Teodoro Duarte Garcia | |
| dc.contributor.author | El-Guendouz, Soukaina | |
| dc.contributor.author | Neves, Rafaela | |
| dc.contributor.author | Duarte, Andreia | |
| dc.contributor.author | Rodrigues, Miguel A. | |
| dc.contributor.author | Pinho Melo, Eduardo | |
| dc.date.accessioned | 2025-02-13T09:27:02Z | |
| dc.date.available | 2025-02-13T09:27:02Z | |
| dc.date.issued | 2024-11-03 | |
| dc.description.abstract | Induced pluripotent stem cells (iPSCs) hold large potential on regenerative medicine due to their pluripotency and unlimited self-renewal capacity without the ethical issues of embryonic stem cells. To provide quality-controlled iPSCs for clinical therapies, it is essential to develop safe cryopreservation protocols for long-term storage, preferable amenable for scale-up and automation. We have compared the impact of two different freezing geometries (bottom-up and conventional radial freezing) on the viability and differentiation capability of human iPSCs. Our results demonstrate that the bottom-up freezing under optimized conditions significantly increases iPSCs viability, up to 9% for the cell membrane integrity and up to 21% for the cell metabolic state, compared to conventional freezing. The improvement achieved for bottom-up versus conventional freezing was maintained after scale-up from cryogenic vials to 30 mL bags, highlighting the method’s potential for clinical applications. These findings show that bottom-up freezing can offer a more controlled and scalable cryopreservation strategy for iPSCs, promoting their future use in regenerative medicine. | eng |
| dc.identifier.doi | 10.1101/2024.10.30.621091 | |
| dc.identifier.issn | 8756-7938 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/26786 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | American Institute of Chemical Engineers (AIChE) | |
| dc.rights.uri | N/A | |
| dc.subject | Cryopreservation | |
| dc.subject | Bottom-up freezing | |
| dc.subject | Induced pluripotent stem cells | |
| dc.subject | Scale- up | |
| dc.title | Enhancing cryopreservation of human iPSCs: Bottom-up vs Conventional freezing geometry | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.title | Biotechnology Progress | |
| oaire.version | http://purl.org/coar/version/c_b1a7d7d4d402bcce | |
| person.familyName | Morais | |
| person.familyName | El-Guendouz | |
| person.familyName | Pinho Melo | |
| person.givenName | Fernando Jorge Teodoro Duarte Garcia | |
| person.givenName | Soukaina | |
| person.givenName | Eduardo | |
| person.identifier | https://scholar.google.com/citations?user=CYBI8q8AAAAJ&hl=fr | |
| person.identifier | 1443188 | |
| person.identifier.ciencia-id | 2517-06F1-0746 | |
| person.identifier.ciencia-id | 3E11-53A1-4D90 | |
| person.identifier.ciencia-id | 3C1C-C10C-1510 | |
| person.identifier.orcid | 0000-0001-5384-1469 | |
| person.identifier.orcid | 0000-0002-3341-9452 | |
| person.identifier.orcid | 0000-0002-0974-8977 | |
| person.identifier.scopus-author-id | 57190122389 | |
| person.identifier.scopus-author-id | 35566177900 | |
| relation.isAuthorOfPublication | e3e3d75b-fd58-4b79-9ae0-81d77994e748 | |
| relation.isAuthorOfPublication | f6745212-30ad-4c15-af2e-37a2b4f71b1e | |
| relation.isAuthorOfPublication | 5fa1895f-5577-4652-961a-886ec9bf41b1 | |
| relation.isAuthorOfPublication.latestForDiscovery | e3e3d75b-fd58-4b79-9ae0-81d77994e748 |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- Unidirectional versus conventional freezing_with figures.pdf
- Size:
- 611.13 KB
- Format:
- Adobe Portable Document Format
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 3.46 KB
- Format:
- Item-specific license agreed upon to submission
- Description: