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Enhancing cryopreservation of human iPSCs: Bottom-up vs Conventional freezing geometry

2024-11-03Journal article Embargoed access
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dc.contributor.authorTeodoro Duarte Garcia Morais, Fernando Jorge
dc.contributor.authorEl-Guendouz, Soukaina
dc.contributor.authorNeves, Rafaela
dc.contributor.authorDuarte, Andreia
dc.contributor.authorRodrigues, Miguel A.
dc.contributor.authorPinho Melo, Eduardo
dc.date.accessioned2025-02-13T09:27:02Z
dc.date.available2025-02-13T09:27:02Z
dc.date.issued2024-11-03
dc.description.abstractInduced pluripotent stem cells (iPSCs) hold large potential on regenerative medicine due to their pluripotency and unlimited self-renewal capacity without the ethical issues of embryonic stem cells. To provide quality-controlled iPSCs for clinical therapies, it is essential to develop safe cryopreservation protocols for long-term storage, preferable amenable for scale-up and automation. We have compared the impact of two different freezing geometries (bottom-up and conventional radial freezing) on the viability and differentiation capability of human iPSCs. Our results demonstrate that the bottom-up freezing under optimized conditions significantly increases iPSCs viability, up to 9% for the cell membrane integrity and up to 21% for the cell metabolic state, compared to conventional freezing. The improvement achieved for bottom-up versus conventional freezing was maintained after scale-up from cryogenic vials to 30 mL bags, highlighting the method’s potential for clinical applications. These findings show that bottom-up freezing can offer a more controlled and scalable cryopreservation strategy for iPSCs, promoting their future use in regenerative medicine.eng
dc.identifier.doi10.1101/2024.10.30.621091
dc.identifier.issn8756-7938
dc.identifier.urihttp://hdl.handle.net/10400.1/26786
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAmerican Institute of Chemical Engineers (AIChE)
dc.rights.uriN/A
dc.subjectCryopreservation
dc.subjectBottom-up freezing
dc.subjectInduced pluripotent stem cells
dc.subjectScale- up
dc.titleEnhancing cryopreservation of human iPSCs: Bottom-up vs Conventional freezing geometryeng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleBiotechnology Progress
oaire.versionhttp://purl.org/coar/version/c_b1a7d7d4d402bcce
person.familyNameTeodoro Duarte Garcia Morais
person.familyNameEl-Guendouz
person.familyNamePinho Melo
person.givenNameFernando Jorge
person.givenNameSoukaina
person.givenNameEduardo
person.identifierhttps://scholar.google.com/citations?user=CYBI8q8AAAAJ&hl=fr
person.identifier1443188
person.identifier.ciencia-id2517-06F1-0746
person.identifier.ciencia-id3E11-53A1-4D90
person.identifier.ciencia-id3C1C-C10C-1510
person.identifier.orcid0000-0001-5384-1469
person.identifier.orcid0000-0002-3341-9452
person.identifier.orcid0000-0002-0974-8977
person.identifier.scopus-author-id57190122389
person.identifier.scopus-author-id35566177900
relation.isAuthorOfPublicatione3e3d75b-fd58-4b79-9ae0-81d77994e748
relation.isAuthorOfPublicationf6745212-30ad-4c15-af2e-37a2b4f71b1e
relation.isAuthorOfPublication5fa1895f-5577-4652-961a-886ec9bf41b1
relation.isAuthorOfPublication.latestForDiscoverye3e3d75b-fd58-4b79-9ae0-81d77994e748

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