Repository logo
 
Publication

Characterization of osteocalcin (BGP) and matrix gla protein (MGP) fish specific antibodies: validation for immunodetection studies in lower vertebrates

2004Journal article Unknown
Simple item page
dc.contributor.authorSimes, D
dc.contributor.authorWilliamson, M. K.
dc.contributor.authorSchaff, Brian J.
dc.contributor.authorGavaia, Paulo J.
dc.contributor.authorIngleton, P. M.
dc.contributor.authorPrice, P. A.
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-25T12:08:18Z
dc.date.available2014-06-25T12:08:18Z
dc.date.issued2004
dc.date.updated2014-05-30T10:28:43Z
dc.description.abstractIn fish species the basic mechanisms of bone development and bone remodeling are not fully understood. The classification of bone tissue in teleosts as cellular or acellular and the presence of transitional states between bone and cartilage and the finding of different types of cartilage in teleosts not previously recognized in higher vertebrates emphasizes the need for a study on the accumulation of the Gla-containing proteins MGP and BGP at the cellular level. In the present study, polyclonal antibodies developed against BGP and MGP from A. regius (a local marine teleost fish) and against MGP from G. galeus (a Pacific Ocean shark), were tested by Western blot for their specificity against BGP and MGP from several other species of teleost fish and shark. For this purpose we extracted and purified both proteins from various marine and freshwater teleosts, identified them by N-terminal amino acid sequence analysis and confirmed the presence of gamma- carboxylation in the proteins with the use of a stain specific for Gla residues. Each antibody recognized either BGP or MGP with no cross-reaction between proteins detected. All purified fish BGPs and MGPs tested were shown to be specifically recognized, thus validating the use of these antibodies for further studies.por
dc.description.sponsorshipPraxis BIA 469/94; PRODEP; BIA 11159/98
dc.identifier.citationD.C. Simes, M.K. Williamson, B.J. Schaff, P.J. Gavaia, P. Ingleton, P.A. Price, M.L. Cancela. 2004. Characterization of Osteocalcin (BGP) and Matrix Gla Protein (MGP) Fish Specific Antibodies: Validation for Immunodetection Studies in Lower Vertebrates. Calcified Tissue International 74:170 –180.por
dc.identifier.doihttp://dx.doi.org/10.1007/s00223-003-0079-4
dc.identifier.otherAUT: LCA00739; AUT: DSI00813;
dc.identifier.urihttp://hdl.handle.net/10400.1/4492
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relationInvolvement of Gla proteins MGP and Osteocalcin in the processes of mineralization occurring in marine versus fresh water teleosts.
dc.titleCharacterization of osteocalcin (BGP) and matrix gla protein (MGP) fish specific antibodies: validation for immunodetection studies in lower vertebratespor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInvolvement of Gla proteins MGP and Osteocalcin in the processes of mineralization occurring in marine versus fresh water teleosts.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCTI%2FCVT%2F42098%2F2001/PT
oaire.citation.endPage180por
oaire.citation.startPage170por
oaire.citation.titleCalcified Tissue Internationalpor
oaire.citation.volume74por
oaire.fundingStreamPOCI
person.familyNameSimes
person.familyNameGavaia
person.familyNameCancela
person.givenNameDina
person.givenNamePaulo
person.givenNameM. Leonor
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0002-5145-4753
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridN-2789-2014
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id7201884723
person.identifier.scopus-author-id6507104377
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication31bac96d-1d01-4b8f-8f2b-9da4be31ea41
relation.isAuthorOfPublication9dca2139-21a4-4d59-aaf7-531f1033a58e
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscoveryb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isProjectOfPublicationf6b77973-b31d-4b6d-bb5b-262bdad3dd5c
relation.isProjectOfPublication.latestForDiscoveryf6b77973-b31d-4b6d-bb5b-262bdad3dd5c

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Characterization of Osteocalcin (BGP) and Matrix Gla Protein (MGP) Fish Specific Antibodies Validation for Immunodetection Studies in Lower Vertebrates.pdf
Size:
280.66 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)