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Disclosing the bioactive metabolites involved in the in vitro anthelmintic effects of salt-tolerant plants through a combined approach using PVPP and HPLC-ESI-MSn

2021-12Journal article Open access
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dc.contributor.authorOliveira, Marta
dc.contributor.authorLima, Caroline Sprengel
dc.contributor.authorKetavong, Setha
dc.contributor.authorLlorent-Martínez, Eulogio J.
dc.contributor.authorHoste, Hervé
dc.contributor.authorCustódio, Luísa
dc.date.accessioned2022-01-13T12:05:42Z
dc.date.available2022-01-13T12:05:42Z
dc.date.issued2021-12
dc.description.abstractStrategies to reduce dependence on synthetic drugs for the treatment of gastrointestinal nematodes (GIN) infections in ruminants include the search for novel anthelmintic scafolds on plants, yet salt-tolerant plants remain overlooked. This study aims to evaluate the in vitro anthelmintic properties of selected salt-tolerant plants against GIN, and identify the potential bioactive secondary metabolites involved. For that purpose, 80% acetone/water extracts were prepared from dried biomass of aerial organs of nine salt-tolerant plant species and tested against Haemonchus contortus and Trichostrongylus colubriformis by the Larval Exsheathment Inhibition Assay (LEIA) and Egg Hatching Inhibition Assay (EHIA). Pistacia lentiscus, Limoniatrum monopetalum, Cladium mariscus and Helychrisum italicum picardi were the most active in both GIN and life stages. To investigate the role of polyphenols in the anthelmintic activity, four selected extracts were treated with polyvinylpolypyrrolidone (PVPP), and non-treated and treated samples were further characterized by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn). While polyphenols seem responsible for the EHIA properties, they are partially accountable to LEIA results. Several phenolics involved in the anthelmintic efects were identifed and discussed. In sum, these species are rich sources of anthelmintic compounds and, therefore, are of major interest for nutraceutical and/or phytotherapeutic applications against GIN in ruminants.pt_PT
dc.description.sponsorship37956XD; 2019-SE2-16; 88881.187940/2018-01
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/s41598-021-03472-9pt_PT
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.1/17477
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Researchpt_PT
dc.relationUnlocking the potential of halophyte plants from extreme maritime environments as sources of innovative products for use in veterinary parasitology
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHaemonchus-Contortustrichopt_PT
dc.subjectTrichostrongyus-Colubriformispt_PT
dc.subjectPistacia-Lentiscopt_PT
dc.subjectNematoides gastrointestinaispt_PT
dc.subjectCompostos fenólicospt_PT
dc.subjectLarvas do 3º estágiopt_PT
dc.subjectLarvas infecciosaspt_PT
dc.subjectExtratospt_PT
dc.subjectAntioxidantept_PT
dc.subjectExelasthmentpt_PT
dc.titleDisclosing the bioactive metabolites involved in the in vitro anthelmintic effects of salt-tolerant plants through a combined approach using PVPP and HPLC-ESI-MSnpt_PT
dc.title.alternativeDivulgação dos metabólitos bioativos envolvidos nos efeitos anténticos in vitro de plantas tolerantes ao sal através de uma abordagem combinada usando PVPP e HPLC-ESI-MSnpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleUnlocking the potential of halophyte plants from extreme maritime environments as sources of innovative products for use in veterinary parasitology
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F123658%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F00425%2F2017%2FCP1391%2FCT0001/PT
oaire.citation.issue1pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStreamOE
oaire.fundingStreamCEEC IND 2017
person.familyNameOliveira
person.familyNameCustódio
person.givenNameMarta
person.givenNameLuísa
person.identifier.ciencia-idC618-E728-0B4C
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.orcid0000-0001-6793-8026
person.identifier.orcid0000-0003-4338-7703
person.identifier.ridM-6101-2013
person.identifier.scopus-author-id15831018900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationf9cfed0f-6b67-413e-988c-ac7397183471
relation.isAuthorOfPublication.latestForDiscovery6b873feb-d39e-45b5-a7be-3c190990843d
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