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Inhalable antitubercular therapy mediated by locust bean gum microparticles

2016-06Journal article Open access
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dc.contributor.authorAlves, Ana
dc.contributor.authorCavaco, Joana
dc.contributor.authorGuerreiro, Filipa
dc.contributor.authorLourenço, J. P.
dc.contributor.authorCosta, Ana M. Rosa da
dc.contributor.authorGrenha, Ana
dc.date.accessioned2016-06-07T14:31:28Z
dc.date.available2016-06-07T14:31:28Z
dc.date.issued2016-06
dc.description.abstractTuberculosis remains a major global health problem and alternative therapeutic approaches are needed. Considering the high prevalence of lung tuberculosis (80% of cases), the pulmonary delivery of antitubercular drugs in a carrier system capable of reaching the alveoli, being recognised and phagocytosed by alveolar macrophages (mycobacterium hosts), would be a significant improvement to current oral drug regimens. Locust bean gum (LBG) is a polysaccharide composed of galactose and mannose residues, which may favour specific recognition by macrophages and potentiate phagocytosis. LBG microparticles produced by spray-drying are reported herein for the first time, incorporating either isoniazid or rifabutin, first-line antitubercular drugs (association efficiencies >82%). Microparticles have adequate theoretical properties for deep lung delivery (aerodynamic diameters between 1.15 and 1.67 μm). The cytotoxic evaluation in lung epithelial cells (A549 cells) and macrophages (THP-1 cells) revealed a toxic effect from rifabutin-loaded microparticles at the highest concentrations, but we may consider that these were very high comparing with in vivo conditions. LBG microparticles further evidenced strong ability to be captured by macrophages (percentage of phagocytosis >94%). Overall, the obtained data indicated the potential of the proposed system for tuberculosis therapy.pt_PT
dc.identifier.doihttps://dx.doi.org/10.3390/molecules21060702pt_PT
dc.identifier.issn1420-3049
dc.identifier.otherAUT: JLO01215; AMG02212; AMC01695;
dc.identifier.urihttp://hdl.handle.net/10400.1/8390
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.subjectLocust bean gumpt_PT
dc.subjectTuberculosis therapypt_PT
dc.subjectSpray-dryingpt_PT
dc.subjectMicroparticlespt_PT
dc.subjectAlveolar macrophagespt_PT
dc.subjectAntitubercular drugspt_PT
dc.subjectInhalable therapypt_PT
dc.titleInhalable antitubercular therapy mediated by locust bean gum microparticlespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue6pt_PT
oaire.citation.startPage702pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume21pt_PT
person.familyNameGuerreiro
person.familyNameLourenço
person.familyNameRosa da Costa
person.familyNameGrenha
person.givenNameFilipa
person.givenNameJoão P.
person.givenNameAna M
person.givenNameAna
person.identifier634248
person.identifier305029
person.identifier.ciencia-idBF1D-7EFA-19BC
person.identifier.ciencia-idF81F-A1F0-2A7C
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.ciencia-id091C-0D58-7225
person.identifier.orcid0000-0001-6224-1553
person.identifier.orcid0000-0002-0501-6672
person.identifier.orcid0000-0003-0225-9537
person.identifier.orcid0000-0002-2136-1396
person.identifier.ridA-7758-2008
person.identifier.ridE-2165-2012
person.identifier.ridH-1392-2017
person.identifier.scopus-author-id57195730600
person.identifier.scopus-author-id53986075100
person.identifier.scopus-author-id8607930100
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationf66ab886-d989-4c1d-9550-527507723c26
relation.isAuthorOfPublication0ca8c16f-6881-4662-bafc-cc51c5ce8d88
relation.isAuthorOfPublication1bd4d8f1-40e5-45af-b1e8-4b8e73a6a70d
relation.isAuthorOfPublication.latestForDiscoveryf66ab886-d989-4c1d-9550-527507723c26

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