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Spatio-temporal dynamics of early somite segmentation in the chicken embryo

2024-04-18Journal article Open Access
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dc.contributor.authorMaia-Fernandes, Ana C
dc.contributor.authorMartins, Ana
dc.contributor.authorBorralho Martins, Nísia
dc.contributor.authorPais de Azevedo, Tomás
dc.contributor.authorMagno, Ramiro
dc.contributor.authordos Santos Duarte, Guilhermina Isabel
dc.contributor.authorAndrade, Raquel
dc.contributor.editorM
dc.date.accessioned2024-09-13T10:32:22Z
dc.date.available2024-09-13T10:32:22Z
dc.date.issued2024-04-18
dc.description.abstractDuring vertebrate embryo development, the body is progressively segmented along the anterior-posterior (A-P) axis early in development. The rate of somite formation is controlled by the somitogenesis embryo clock (EC), which was first described as gene expression oscillations of hairy1 (hes4) in the presomitic mesoderm of chick embryos with 15-20 somites. Here, the EC displays the same periodicity as somite formation, 90 min, whereas the posterior-most somites (44-52) only arise every 150 minutes, matched by a corresponding slower pace of the EC. Evidence suggests that the rostral-most somites are formed faster, however, their periodicity and the EC expression dynamics in these early stages are unknown. In this study, we used time-lapse imaging of chicken embryos from primitive streak to somitogenesis stages with high temporal resolution (3-minute intervals). We measured the length between the anterior-most and the last formed somitic clefts in each captured frame and developed a simple algorithm to automatically infer both the length and time of formation of each somite. We found that the occipital somites (up to somite 5) form at an average rate of 75 minutes, while somites 6 onwards are formed approximately every 90 minutes. We also assessed the expression dynamics of hairy1 using half-embryo explants cultured for different periods of time. This showed that EC hairy1 expression is highly dynamic prior to somitogenesis and assumes a clear oscillatory behaviour as the first somites are formed. Importantly, using ex ovo culture and live-imaging techniques, we showed that the hairy1 expression pattern recapitulates with the formation of each new pair of somites, indicating that somite segmentation is coupled with EC oscillations since the onset of somitogenesis.eng
dc.description.sponsorshipSFRH/BD/146043/2019; 2021.00238.CEECIND
dc.identifier.doi10.1371/journal.pone.0297853
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.1/25881
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPublic Library of Science (PLoS)
dc.relationTIMING CELL DIFFERENTIATON: Gene Regulatory Network in Temporal Control of Mesoderm Specification
dc.relationHealth Research Network: From Lab to Community Health
dc.relation.ispartofPLOS ONE
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleSpatio-temporal dynamics of early somite segmentation in the chicken embryoeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTIMING CELL DIFFERENTIATON: Gene Regulatory Network in Temporal Control of Mesoderm Specification
oaire.awardTitleHealth Research Network: From Lab to Community Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBEX-BID%2F5410%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0053%2F2020/PT
oaire.citation.issue4
oaire.citation.startPagee0297853
oaire.citation.titlePLoS ONE
oaire.citation.volume19
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameMaia-Fernandes
person.familyNameMartins
person.familyNameBorralho Martins
person.familyNamePais de Azevedo
person.familyNameMagno
person.familyNamedos Santos Duarte
person.familyNameAndrade
person.givenNameAna C
person.givenNameAna
person.givenNameNísia
person.givenNameTomás
person.givenNameRamiro
person.givenNameGuilhermina Isabel
person.givenNameRaquel
person.identifier1418026
person.identifier.ciencia-idE113-84F0-5FB7
person.identifier.ciencia-id1C18-6533-49F5
person.identifier.ciencia-idED15-7F67-7B64
person.identifier.ciencia-id2312-360B-227A
person.identifier.orcid0000-0002-5086-2632
person.identifier.orcid0000-0002-3304-6051
person.identifier.orcid0000-0001-5713-3240
person.identifier.orcid0000-0002-3239-2198
person.identifier.orcid0000-0001-5226-3441
person.identifier.orcid0000-0003-0060-2936
person.identifier.orcid0000-0002-0397-5917
person.identifier.scopus-author-id17344059400
person.identifier.scopus-author-id55308096300
person.identifier.scopus-author-id7103114918
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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