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Assessment of larval quality of two bivalve species, Crassostrea angulata and Chamelea gallina, exposed and cryopreserved with different cryoprotectant solutions

2022Journal article Restricted access
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dc.contributor.authorAnjos, Catarina
dc.contributor.authorDuarte, Daniel
dc.contributor.authorDiogo, Patrícia
dc.contributor.authorMatias, Domitília
dc.contributor.authorCabrita, Elsa
dc.date.accessioned2022-12-13T10:44:45Z
dc.date.available2022-12-13T10:44:45Z
dc.date.issued2022
dc.description.abstractMarine bivalves are valuable resources, however, some shellfish populations are endangered due to factors such as anthropogenic pressure, pathologies or lack of reproduction synchrony. Portuguese oyster (Crassostrea angulata) and striped venus clam (Chamelea gallina) have high socio-economic value and their endangered natural populations require rehabilitation. Cryopreservation is a valuable method for the preservation and management of genetic resources for aquaculture and restocking. Larvae cryopreservation is particularly valuable since diploid organisms are obtained upon thawing. The objective of this work was the establishment of C. angulata and C. gallina D-larvae cryopreservation through the selection of permeant cryoprotectant in the freezing solution, namely ethylene glycol (EG) and dimethyl sulfoxide (Me2SO). Cryoprotectants exposure showed that, in C. angulata, Me2SO promoted significantly higher incidence of abnormalities and enhanced glutathione reductase activity when compared to control (larvae without cryoprotectant exposure) or even to EG treatment. However, for both species, EG significantly reduced D-larvae average path velocity (VAP). In C. angulata post-thaw D-larvae, EG treatment promoted significantly lower motility and velocity when compared to control and Me2SO treatment. Superoxide dismutase (SOD) activity showed a reduction in C. angulata postthaw D-larvae when compared to control, which was compensated by the enhancement of glutathione peroxidase (GPX) activity. In C. gallina post-thaw D-larvae, only motility, velocity and SOD activity were significantly lower than control. Therefore, the best treatment to cryopreserve C. angulata D-larvae was EG while for C. gallina Me2SO produced better results. This work established for the first time D-larvae cryopreservation protocols for C. angulata and C. gallina.pt_PT
dc.description.sponsorshipCCMAR/Multi/04326/2022
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.cryobiol.2022.04.007pt_PT
dc.identifier.issn0011-2240
dc.identifier.urihttp://hdl.handle.net/10400.1/18630
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAssociation of European Marine Biological Laboratories Expanded
dc.relationEstablishment of a genetic resource bank for restocking management in Portuguese oyster Crassostrea angulata and Striped-venus clam Chamelea gallina
dc.subjectOysterpt_PT
dc.subjectClampt_PT
dc.subjectD-larvaept_PT
dc.subjectCryopreservationpt_PT
dc.subjectEthylene glycolpt_PT
dc.subjectDimethyl sulfoxidept_PT
dc.titleAssessment of larval quality of two bivalve species, Crassostrea angulata and Chamelea gallina, exposed and cryopreserved with different cryoprotectant solutionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAssociation of European Marine Biological Laboratories Expanded
oaire.awardTitleEstablishment of a genetic resource bank for restocking management in Portuguese oyster Crassostrea angulata and Striped-venus clam Chamelea gallina
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/730984/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F130910%2F2017/PT
oaire.citation.endPage31pt_PT
oaire.citation.startPage24pt_PT
oaire.citation.titleCryobiologypt_PT
oaire.citation.volume106pt_PT
oaire.fundingStreamH2020
person.familyNameAnjos
person.familyNameCabrita
person.givenNameCatarina
person.givenNameElsa
person.identifier.ciencia-id3B1A-C5A1-E96C
person.identifier.orcid0000-0002-2038-2187
person.identifier.orcid0000-0003-3864-8841
person.identifier.ridE-8523-2012
person.identifier.scopus-author-id26645697000
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationacc899b9-bc7a-4e9c-a7ad-1226c32c5da3
relation.isAuthorOfPublication332fa9b9-09b5-47fe-a1b3-afe088b4150a
relation.isAuthorOfPublication.latestForDiscoveryacc899b9-bc7a-4e9c-a7ad-1226c32c5da3
relation.isProjectOfPublicationa6bab18e-16a5-48af-8699-7a2bef8b6fac
relation.isProjectOfPublicationbaf93aa0-5d14-44db-813a-2a39c5eb83aa
relation.isProjectOfPublication.latestForDiscoverya6bab18e-16a5-48af-8699-7a2bef8b6fac

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