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Isolation of highly polymorphic microsatellite loci for a species with a large genome size: Sharp-ribbed salamander (Pleurodeles waltl)

2009Journal article Restricted access
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dc.contributor.authorVan De Vliet, M. S.
dc.contributor.authorDiekmann, O. E.
dc.contributor.authorSerrão, Ester
dc.contributor.authorBeja, Pedro
dc.date.accessioned2014-06-02T15:28:48Z
dc.date.available2014-06-02T15:28:48Z
dc.date.issued2009
dc.date.updated2014-05-21T13:24:47Z
dc.description.abstractFourteen highly polymorphic microsatellite markers were developed and characterized for the sharp-ribbed salamander, Pleurodeles waltl. Isolating microsatellites with more than 12 single repeat type units was only successful for a tetranucleotide repeat (ATAG). Compared to microsatellite libraries constructed simultaneously for two anuran amphibian species, a greater number of primer pairs designed for P. waltl had to be discarded, due to consistent amplification problems. Low amplification success rate for P. waltl may be due to its larger genome size. Consequently, to avoid nonspecific binding and to increase amplification success, polymerase chain reaction programmes with touchdown cycles were used. For 14 microsatellite markers, amplification was successful and consistent with number of alleles and expected heterozygosity ranging from seven to 22 and from 0.79 to 0.94, respectively. All 14 microsatellite markers will be extremely useful for metapopulation studies of this unique amphibian species.por
dc.identifier.citationVan De Vliet, M.S.; Diekmann, O.E.; Serrão, E.A.; Beja, P. Isolation of highly polymorphic microsatellite loci for a species with a large genome size: Sharp-ribbed salamander (Pleurodeles waltl), Molecular Ecology Resources, 9, 1, 425-428, 2009.por
dc.identifier.doihttp://dx.doi.org/10.1111/j.1755-0998.2008.02436.x
dc.identifier.issn1755-098X
dc.identifier.otherAUT: ESE00527;
dc.identifier.urihttp://hdl.handle.net/10400.1/4149
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.subjectAmplification successpor
dc.subjectLarge genome sizepor
dc.subjectMicrosatellitespor
dc.subjectPleurodeles waltlpor
dc.subjectSsharp-ribbed salamanderpor
dc.titleIsolation of highly polymorphic microsatellite loci for a species with a large genome size: Sharp-ribbed salamander (Pleurodeles waltl)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage428por
oaire.citation.issue1por
oaire.citation.startPage425por
oaire.citation.titleMolecular Ecology Resourcespor
oaire.citation.volume9por
person.familyNameSerrao
person.familyNameCorreia de Oliveira Beja
person.givenNameEster A.
person.givenNamePedro Rui
person.identifierC-6686-2012
person.identifier6xecf34AAAAJ
person.identifier.ciencia-id5B13-B26E-B1EC
person.identifier.ciencia-idC116-3331-F9E6
person.identifier.orcid0000-0003-1316-658X
person.identifier.orcid0000-0001-8164-0760
person.identifier.scopus-author-id7004093604
person.identifier.scopus-author-id6602807125
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication45ccfe90-155c-4d6f-9e86-8f0fd064005f
relation.isAuthorOfPublication74f734df-0ea1-43de-9ed7-d0c731ab7c07
relation.isAuthorOfPublication.latestForDiscovery45ccfe90-155c-4d6f-9e86-8f0fd064005f

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