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Ca2+-Calmodulin regulation of testicular androgen production in Mozambique tilapia (Oreochromis mossambicus)

2009Journal article Restricted access
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dc.contributor.authorMartins, Rute S. T.
dc.contributor.authorFuentes, J.
dc.contributor.authorAlmeida, O. G.
dc.contributor.authorPower, Deborah
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-09T09:07:43Z
dc.date.available2014-05-09T09:07:43Z
dc.date.issued2009
dc.date.updated2014-05-08T10:41:29Z
dc.description.abstractThe Ca2+-Calmodulin (CaM) signaling pathway has previously been shown to be involved in the regulation of teleost fish ovarian steroidogenesis. However, a putative role of CaM in testicular steroidogenesis and potential targets has not been examined. To examine whether basal steroidogenesis is modulated by Ca2+ and CaM levels in the testis of Mozambique tilapia (Oreochromis mossambicus) we have incubated testicular fragments in vitro under different conditions and analyzed steroid output. Calcium-free medium with or without EGTA did not affect testicular basal 11-ketotestosterone (11-KT) and testosterone (T) secretion. However, addition of 80 lM the CaM inhibitor W7 significantly reduced basal 11-KT, T and androstenedione secretion. Interestingly, the decreased androgen production by 80 lM of W7 was accompanied by increased 11-desoxicortisol output and by the activation of cortisol synthesis in the testis, the latter undetected in untreated tissues. However, production of 17,20a-dihydroxy-4-pregnen- 3-one was unaltered by W7. This suggests that C17,20 desmolase, 21-hydroxylase and possibly 11bhydroxysteroid dehydrogenase are targets for CaM. In addition, androgen production was also found to be regulated by the level of cAMP since incubations with forskolin (FK) significantly increased 11-KT and T output. A cross-talk between the cAMP and Ca2+-CaM signaling pathways was detected since W7 administration also decreased FK stimulated androgen production. Altogether, these data show that both basal and cAMP stimulated androgen levels were modulated by intracellular Ca2+-dependent CaM and that possibly Ca2+-CaM determines the shift in steroidogenesis from C21 steroids to androgens.por
dc.identifier.citationMartins, Rute S.T.; Fuentes, Juan; Almeida, Olinda; Power, Deborah M.; Canario, Adelino V.M. Ca2+-Calmodulin regulation of testicular androgen production in Mozambique tilapia (Oreochromis mossambicus), General and Comparative Endocrinology, 162, 2, 153-159, 2009.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygcen.2009.03.017
dc.identifier.issn0016-6480
dc.identifier.otherAUT: DPO00386; ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3814
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectCa2+-Calmodulinpor
dc.subjectcAMPpor
dc.subjectTestosteronepor
dc.subject11-Ketotestosteronepor
dc.subjectCortisolpor
dc.subjectTestispor
dc.subjectTilapiapor
dc.titleCa2+-Calmodulin regulation of testicular androgen production in Mozambique tilapia (Oreochromis mossambicus)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage159por
oaire.citation.issue2por
oaire.citation.startPage153por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume162por
person.familyNameFuentes
person.familyNamePower
person.familyNameCanario
person.givenNameJuan
person.givenNameDeborah Mary
person.givenNameAdelino
person.identifier392998
person.identifier143624
person.identifier.ciencia-id421B-E196-2C33
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0003-0430-8734
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7201832526
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationd354b937-b18c-4ca5-a843-04a04269d60f
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
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relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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