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Responsiveness of pituitary to galanin throughout the reproductive cycle of male European sea bass (Dicentrarchus labrax)

2017-09Journal article Open Access
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dc.contributor.authorPinto, P.
dc.contributor.authorVelez, Zélia
dc.contributor.authorSousa, Carmen
dc.contributor.authorSantos, Soraia
dc.contributor.authorAndrade, André
dc.contributor.authorAlvarado, M. V.
dc.contributor.authorFelip, A.
dc.contributor.authorZanuy, S.
dc.contributor.authorCanario, A.V.M.
dc.date.accessioned2019-11-20T15:07:24Z
dc.date.available2019-11-20T15:07:24Z
dc.date.issued2017-09
dc.description.abstractThe neuropeptide galanin (Gal) is a putative factor regulating puberty onset and reproduction through its actions on the pituitary. The present study investigated the pituitary responsiveness to galanin and the patterns of galanin receptors (Galrs) expression throughout the reproductive cycle of two years old male European sea bass (Dicentrarchus labrax), an important aquaculture species. Quantitative analysis of pituitary and hypothalamus transcript expression of four galr subtypes revealed differential regulation according to the testicular developmental stage, with an overall decrease in expression from the immature stage to the mid-recrudescence stage. Incubation of pituitary cells with mammalian 1-29 Gal peptide induced significant changes in cAMP concentration, with sensitivities that varied according to the testicular development stages. Furthermore 1-29 Gal was able to stimulate both follicle stimulating hormone (Fsh) and luteinizing hormone (Lh) release from pituitary cell suspensions. The magnitude of the effects and effective concentrations varied according to reproductive stage, with generalized induction of Fsh and Lh release in animals sampled in January (full spermiation). The differential expression of galrs in pituitary and hypothalamus across the reproductive season, together with the differential effects of Gal on gonadotropins release in vitro strongly suggests the involvement of the galaninergic system in the regulation the hypothalamus-pituitary-gonad axis of male sea bass. This is to our knowledge the first clear evidence for the involvement of galanin in the regulation of reproduction in non-mammalian vertebrates. (C) 2017 Elsevier Inc. All rights reserved.
dc.description.sponsorshipEuropean Union Seventh Framework Programme [262336]
dc.description.sponsorshipSpanish Ministry of Science and Innovation (MICINN)
dc.description.sponsorshipSpanish Ministry of the Economy and Competitiveness (MINECO) [AGL2009-11086]
dc.description.sponsorshipSpanish Ministry of the Economy and Competitiveness
dc.description.sponsorshipRegional Government of Valencia [PROME-TEOH/2014/051]
dc.description.versioninfo:eu-repo/semantics/acceptedVersion
dc.identifier.doi10.1016/j.ygcen.2017.06.009
dc.identifier.issn0016-6480
dc.identifier.issn1095-6840
dc.identifier.urihttp://hdl.handle.net/10400.1/13025
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAcademic Press Inc Elsevier Science
dc.rights.urihttp://creativecommons.org/licenses/byncnd/4.0/
dc.subjectLuteinizing-hormone secretion
dc.subjectGene-expression
dc.subjectTeleost fish
dc.subjectSexual-dimorphism
dc.subjectBinding-sites
dc.subjectLh-release
dc.subjectAutoradiographic distribution
dc.subjectImmunoreactive neurons
dc.subjectCarassius-auratus
dc.subjectKnockout mice
dc.titleResponsiveness of pituitary to galanin throughout the reproductive cycle of male European sea bass (Dicentrarchus labrax)
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F84033%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR-BIO%2F3890%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.citation.endPage161
oaire.citation.startPage152
oaire.citation.titleGeneral and Comparative Endocrinology
oaire.citation.volume250
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
person.familyNamePinto
person.familyNameVelez
person.familyNameVieira de Sousa
person.familyNameSantos
person.familyNameAlçada Baptista Rebelo de Andrade
person.familyNameCanario
person.givenNamePatricia IS
person.givenNameZélia
person.givenNameCármen Sofia
person.givenNameSoraia
person.givenNameAndré
person.givenNameAdelino
person.identifier643457
person.identifier143624
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person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0003-2761-4048
person.identifier.orcid0000-0002-3483-1932
person.identifier.orcid0000-0001-5006-2021
person.identifier.orcid0000-0002-5900-8782
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridM-3817-2013
person.identifier.ridM-5283-2013
person.identifier.ridC-1328-2018
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id16176444400
person.identifier.scopus-author-id56568523700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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