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Analysis of genetic diversity in Portuguese Ceratonia siliqua L. cultivars using RAPD and AFLP markers

dc.contributor.authorBarracosa, Paulo
dc.contributor.authorLima, M. B.
dc.contributor.authorCravador, A.
dc.date.accessioned2012-05-14T14:48:04Z
dc.date.available2012-05-14T14:48:04Z
dc.date.issued2008
dc.date.updated2012-05-12T23:16:39Z
dc.description.abstractAlthough carob (Ceratonia siliqua L.) is of great economic importance little is still known about the pattern of genetic variation within this species. Morphological characteristics based on 31 fruit and seeds of continuous characters determinant for agro-industrial uses, were compared with RAPD and AFLP markers for assessing genetic distances in 68 accessions of carob trees, from different cultivars, varieties and eco-geographic regions of Algarve. Eighteen selected RAPD primers applied to the 68 accessions produced a total of 235 fragments ranging from 200 to 2000 bp, of which 93 (40%) were polymorphic. Four AFLP selective primer combinations generated a total of 346 amplification fragments of which 110 were polymorphic. The average level of polymorphism based on four primer combinations was 31.8%. The phenetic trees based on RAPD and AFLP analyses gave high co-phenetic correlation values, and were found to be consistent in general with the analysis of morphological data, carried out on the same accessions. A number of RAPD and AFLP markers were found to be diagnostic for ‘Canela’ cultivar and 13 wild ungrafted trees.por
dc.identifier.citationBarracosa, Paulo; Lima, M.B.; Cravador, Alfredo. Analysis of genetic diversity in Portuguese Ceratonia siliqua L. cultivars using RAPD and AFLP markers. Scientia Horticulturae, 118, 3, 189-199, 2008.por
dc.identifier.issn0304-4238
dc.identifier.otherAUT: ACR00659;
dc.identifier.urihttp://hdl.handle.net/10400.1/1163
dc.language.isoengpor
dc.subjectRAPDpor
dc.subjectAFLPpor
dc.subjectGenetic diversitypor
dc.subjectCarobpor
dc.subjectCeratonia siliquapor
dc.subjectDieta mediterrânicapt_PT
dc.titleAnalysis of genetic diversity in Portuguese Ceratonia siliqua L. cultivars using RAPD and AFLP markerspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage199por
oaire.citation.issue118(3)por
oaire.citation.startPage189por
oaire.citation.titleThe differential expression of four Phytophthora cinnamomi elicitin genes was analysed by Real Timepor
person.familyNameCravador
person.givenNameAlfredo
person.identifier.orcid0000-0002-9831-9815
person.identifier.ridK-7247-2012
person.identifier.scopus-author-id6602537257
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication8c84a9e6-6b7f-4b50-93a2-152c85901722
relation.isAuthorOfPublication.latestForDiscovery8c84a9e6-6b7f-4b50-93a2-152c85901722

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