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Glucose overload in yolk has little effect on the long-term modulation of carbohydrate metabolic genes in zebrafish (Danio rerio)

2014Journal article Open access
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dc.contributor.authorRocha, Filipa
dc.contributor.authorDias, J.
dc.contributor.authorEngrola, S.
dc.contributor.authorGavaia, Paulo J.
dc.contributor.authorGeurden, Inge
dc.contributor.authorDinis, Maria Teresa
dc.contributor.authorPanserat, S.
dc.date.accessioned2014-06-05T13:06:15Z
dc.date.available2014-06-05T13:06:15Z
dc.date.issued2014
dc.date.updated2014-05-30T09:49:59Z
dc.description.abstractThe use of early nutritional stimuli to program metabolic pathways in fish is ill defined. Therefore, studies were undertaken with zebrafish to assess the effect of high glucose levels during the embryonic stage as a lifelong modulator of genes involved in carbohydrate metabolism. Genes related to carbohydrate metabolism were expressed at low levels at 0.2 and 1 day post-fertilization (dpf). However, from 4 dpf onwards there was a significant increase on expression of all genes, suggesting that all analysed pathways were active. By microinjection, we successfully enriched zebrafish egg yolk with glucose (a 43-fold increase of basal levels). Acute effects of glucose injection on gene expression were assessed in larvae up to 10 dpf, and the programming concept was evaluated in juveniles (41 dpf) challenged with a hyperglucidic diet. At 4 dpf, larvae from glucose-enriched eggs showed a downregulation of several genes related to glycolysis, glycogenolysis, lipogenesis and carbohydrate digestion in comparison with control (saline-injected) embryos. This inhibitory regulation was suppressed after 10 dpf. At the juvenile stage, and upon switching from a low to a high digestible carbohydrate diet, early glucose enrichment had no significant effect on most analysed genes. However, these same fish showed altered expression of the genes for cytosolic phosphoenolpyruvate carboxykinase, sodium-dependent glucose cotransporter 1 and glycogen synthase, suggesting changes to the glucose storage capacity in muscle and glucose production and transport in viscera. Overall, supplementation of egg yolk with high glucose levels had little effect on the long-term modulation of carbohydrate metabolic genes in zebrafish.por
dc.identifier.citationRocha, Filipa; Dias, Jorge; Engrola, Sofia; Gavaia, Paulo Jorge Travessa; Geurden, Inge; Dinis, Maria Teresa; Panserat, Stephane. Glucose overload in yolk has little effect on the long-term modulation of carbohydrate metabolic genes in zebrafish (Danio rerio), Journal of Experimental Biology, 217, 7, 1139-11, 2014.por
dc.identifier.doihttp://dx.doi.org/10.1242/jeb.095463
dc.identifier.issn0022-0949
dc.identifier.urihttp://hdl.handle.net/10400.1/4195
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherCompany of Biologistspor
dc.subjectGlucose metabolismpor
dc.subjectNutritional programmingpor
dc.subjectGene expressionpor
dc.subjectZebrafishpor
dc.titleGlucose overload in yolk has little effect on the long-term modulation of carbohydrate metabolic genes in zebrafish (Danio rerio)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1149por
oaire.citation.issue7por
oaire.citation.startPage1139por
oaire.citation.titleJournal of Experimental Biologypor
oaire.citation.volume217por
person.familyNameRocha
person.familyNameEngrola
person.familyNameGavaia
person.familyNameDinis
person.givenNameFilipa
person.givenNameSofia
person.givenNamePaulo
person.givenNameMaria Teresa
person.identifier237846
person.identifier.ciencia-idA610-29E7-9B83
person.identifier.ciencia-idB619-FC16-D007
person.identifier.ciencia-id5414-4ACF-BF90
person.identifier.orcid0000-0002-7531-9302
person.identifier.orcid0000-0002-5244-5541
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0001-7813-1324
person.identifier.ridA-2485-2012
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id8669620600
person.identifier.scopus-author-id6507104377
person.identifier.scopus-author-id7004150905
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication1db7d79c-3060-4f54-88dd-7c6c48ffa247
relation.isAuthorOfPublication51be5a20-df9c-4a90-85eb-51d779642caa
relation.isAuthorOfPublication9dca2139-21a4-4d59-aaf7-531f1033a58e
relation.isAuthorOfPublication309dec19-8aa9-4bea-8665-42dd4b3a9894
relation.isAuthorOfPublication.latestForDiscovery309dec19-8aa9-4bea-8665-42dd4b3a9894

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