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Sperm parameters and epididymis function in transgenic rats overexpressing the Ca-2-binding protein regucalcin: a hidden role for Ca-2 in sperm maturation?

2013-09Journal article Open access
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dc.contributor.authorCorreia, S.
dc.contributor.authorOliveira, P. F.
dc.contributor.authorGuerreiro, P. M.
dc.contributor.authorLopes, G.
dc.contributor.authorAlves, M. G.
dc.contributor.authorCanario, Adelino
dc.contributor.authorCavaco, J. E.
dc.contributor.authorSocorro, Silvia
dc.date.accessioned2018-12-07T14:58:13Z
dc.date.available2018-12-07T14:58:13Z
dc.date.issued2013-09
dc.description.abstractSperm undergo maturation acquiring progressive motility and the ability to fertilize oocytes through exposure to the components of the epididymal fluid (EF). Although the establishment of a calcium (Ca-2) gradient along the epididymis has been described, its direct effects on epididymal function remain poorly explored. Regucalcin (RGN) is a Ca-2-binding protein, regulating the activity of Ca-2-channels and Ca-2-ATPase, for which a role in male reproductive function has been suggested. This study aimed at comparing the morphology, assessed by histological analysis, and function of epididymis, by analysis of sperm parameters, antioxidant potential and Ca-2 fluxes, between transgenic rats overexpressing RGN (Tg-RGN) and their wild-type littermates. Tg-RGN animals displayed an altered morphology of epididymis and lower sperm counts and motility. Tissue incubation with Ca-45(2) showed also that epididymis of Tg-RGN displayed a diminished rate of Ca-2-influx, indicating unbalanced Ca-2 concentrations in the epididymal lumen. Sperm viability and the frequency of normal sperm, determined by the one-step eosin-nigrosin staining technique and the Diff-Quik staining method, respectively, were higher in Tg-RGN. Moreover, sperm of Tg-RGN rats showed a diminished incidence of tail defects. Western blot analysis demonstrated the presence of RGN in EF as well as its higher expression in the corpus region. The results presented herein demonstrated the importance of maintaining Ca-2-levels in the epididymal lumen and suggest a role for RGN in sperm maturation. Overall, a new insight into the molecular mechanisms driving epididymal sperm maturation was obtained, which could be relevant to development of better approaches in male infertility treatment and contraception.
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1093/molehr/gat030
dc.identifier.issn1360-9947
dc.identifier.urihttp://hdl.handle.net/10400.1/11914
dc.language.isoeng
dc.peerreviewedyes
dc.publisherOxford Univ Press
dc.relationStrategic Project - UI 709 - 2011-2012
dc.relationTYPE 2 DIABETES MELLITUS AND MALE INFERTILITY: UNRAVELING THE METABOLIC DYSFUNCTION IN TESTES AND SERTOLI CELLS
dc.relationREGUCALCIN, A NEW SEX STEROID TARGET GENE AND AN APOPTOSIS REGULATOR IN SPERMATOGENESIS
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSenescence marker protein-30
dc.subjectAcute liver-failure
dc.subjectSignal-transduction
dc.subjectGene-expression
dc.subjectTyrosine phosphorylation
dc.subjectCa2+-atpase Activity
dc.subjectMass-spectrometry
dc.subjectPlasma-Membranes
dc.subjectMale-fertility
dc.subjectCalcium
dc.titleSperm parameters and epididymis function in transgenic rats overexpressing the Ca-2-binding protein regucalcin: a hidden role for Ca-2 in sperm maturation?
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 709 - 2011-2012
oaire.awardTitleTYPE 2 DIABETES MELLITUS AND MALE INFERTILITY: UNRAVELING THE METABOLIC DYSFUNCTION IN TESTES AND SERTOLI CELLS
oaire.awardTitleREGUCALCIN, A NEW SEX STEROID TARGET GENE AND AN APOPTOSIS REGULATOR IN SPERMATOGENESIS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FUI0709%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F60945%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F80451%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F60945%2F2009/PT
oaire.citation.endPage589
oaire.citation.issue9
oaire.citation.startPage581
oaire.citation.titleMolecular Human Reproduction
oaire.citation.volume19
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStreamSFRH
oaire.fundingStreamFARH
oaire.fundingStreamFARH
person.familyNameGuerreiro da Costa Guerreiro
person.familyNameCanario
person.givenNamePedro Miguel
person.givenNameAdelino
person.identifierA-2539-2009
person.identifier143624
person.identifier.ciencia-id5C13-965D-3148
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0001-5371-7919
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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