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Molecular cloning and expression analysis of three omega-6 desaturase genes from purslane (Portulaca oleracea L.)

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dc.contributor.authorTeixeira, Monica
dc.contributor.authorCoelho, N.
dc.contributor.authorOlsson, M. E.
dc.contributor.authorBrodelius, P. E.
dc.contributor.authorCarvalho, Isabel Saraiva de
dc.contributor.authorBrodelius, Maria
dc.date.accessioned2012-11-22T14:07:22Z
dc.date.available2012-11-22T14:07:22Z
dc.date.issued2009
dc.description.abstractTwo full-length cDNA clones of PoleFAD2 and one full-length cDNA clone of PoleFAD6, encoding omega-6 fatty acid desaturases, the key enzymes for the conversion of oleic into linoleic acid, were isolated from purslane (Portulaca oleracea L.) leaves and seeds. The deduced amino acid sequence of both isoforms of PoleFAD2 showed higher similarities to other microsomal omega-6 desaturases then to PoleFAD6 or other plastidial orthologues, and vice versa. Expression analysis by RT-PCR showed that all genes are expressed in all tissues of purslane tested, but higher levels of mRNA accumulation were detected in reproductive organs and cells that proliferate rapidly or store lipids. Wounding affected the levels of mRNA accumulation of both, FAD2 and FAD6 genes in purslane leaves, while chilling stress affected only FAD2 transcript level. The expression patterns observed reflect the discrete roles of these genes in membrane synthesis for cell division, thylakoid development, and lipid storage or in the biosynthetic pathway for the production of signaling molecules that influence plant development or defense.por
dc.identifier.otherAUT: ICA01121;
dc.identifier.urihttp://hdl.handle.net/10400.1/1859
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relation.publisherversionhttp://link.springer.com/10.1007/s10529-009-9956-xpor
dc.subjectFAD2por
dc.subjectFAD6por
dc.subjectFatty acid desaturasespor
dc.subjectPurslanepor
dc.subjectSpatial expressionpor
dc.subjectWoundingpor
dc.subjectChilling stresspor
dc.titleMolecular cloning and expression analysis of three omega-6 desaturase genes from purslane (Portulaca oleracea L.)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1101por
oaire.citation.startPage1089por
oaire.citation.titleBiotechnology Letterspor
oaire.citation.volume31por
person.familyNameCoelho
person.familyNameSaraiva de Carvalho
person.givenNameNatacha
person.givenNameIsabel Maria Marques
person.identifier.ciencia-idC518-BDB3-E127
person.identifier.ciencia-id591A-3777-B036
person.identifier.orcid0000-0003-4265-5622
person.identifier.orcid0000-0001-8057-3404
person.identifier.scopus-author-id35995183800
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication43b2d7ec-049a-412b-ad22-c9db64cc572c
relation.isAuthorOfPublicationaf26e659-578b-4e5f-a649-384cd5e4f8e3
relation.isAuthorOfPublication.latestForDiscovery43b2d7ec-049a-412b-ad22-c9db64cc572c

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