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DNA extraction and RAPD markers to assess the genetic similarity among Gelidium sesquipedale (Rhodophyta) populations

1997Journal article Restricted access
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dc.contributor.authorAlberto, F.
dc.contributor.authorSantos, Rui
dc.contributor.authorLeitão, J. M.
dc.date.accessioned2015-06-19T14:36:27Z
dc.date.available2015-06-19T14:36:27Z
dc.date.issued1997
dc.description.abstractA simple method developed for genomic DNA isolation from fungus was tested on the red alga, Gelidium sesquipedale (Clem.) Born et Thur., which is commercially exploited for its high sulfated polysaccharide (agar) content. This method is faster, cheaper, and less toxic than conventional phenol/chloroform methods. Random amplified polymorphic DNA (RAPD) amplifications were performed successfully without the necessity of purifying the DNA. RAPD markers were used to investigate the genetic similarity among three natural populations of G. sesquipedale from southern Portugal. Bulked-genomic DNA samples of 15 different individuals were made in each population. These can be conceived of as a sample of the population DNA. Of the 62 primers screened, 41 produced bands and 22 revealed polymorphisms. Genetic similarities among populations were high. Populations that are further away from each other have the lowest similarity coefficients, whereas the intermediate Ingrina population, located on the south coast, showed higher genetic similarity with the Odeceixe population located on the southwest coast, than with the Sao Rafael southern population. This suggests a higher genetic flow between Odeceixe and Ingrina or the result may be a founder effect in the sense that the species has propagated from the east coast to the south coast of Portugal. We conclude that the use of this isolation method with RAPD analysis is appropriate to characterize the genetic variability of this commercial species along its geographical distribution. Large sample sizes can be screened at a relatively low cost. Finding genetic markers for commercial populations of C. sesquipedale may be of industrial interest.
dc.identifier.doihttps://dx.doi.org/10.1111/j.0022-3646.1997.00706.x
dc.identifier.issn0022-3646
dc.identifier.otherAUT: JLE00446;
dc.identifier.urihttp://hdl.handle.net/10400.1/6513
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPhycological Society of America
dc.relation.isbasedonP-001-AZG
dc.titleDNA extraction and RAPD markers to assess the genetic similarity among Gelidium sesquipedale (Rhodophyta) populations
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage710
oaire.citation.startPage706
oaire.citation.titleJournal of Phycology
oaire.citation.volume33
person.familyNameAlberto
person.familyNameSantos
person.familyNameLeitão
person.givenNameFilipe
person.givenNameRui
person.givenNameJosé
person.identifier1488456
person.identifier42560
person.identifier258398
person.identifier.ciencia-idBD15-2AC7-8AA3
person.identifier.ciencia-id9B18-444D-2244
person.identifier.ciencia-id7A17-3E0A-73AD
person.identifier.orcid0000-0003-0593-3240
person.identifier.orcid0000-0002-7861-4366
person.identifier.orcid0000-0003-4278-0415
person.identifier.ridB-4168-2008
person.identifier.ridAAH-3958-2020
person.identifier.scopus-author-id6701653422
person.identifier.scopus-author-id7201375018
person.identifier.scopus-author-id7005119796
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication4e95893d-e3c7-462f-a897-49bb8999b65b
relation.isAuthorOfPublication84487ca8-404a-4584-81f0-3662c340d712
relation.isAuthorOfPublication0f990d1c-2913-4f8a-8fb6-f8b02721247f
relation.isAuthorOfPublication.latestForDiscovery84487ca8-404a-4584-81f0-3662c340d712

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