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Mir-20a regulates in vitro mineralization and BMP signaling pathway by targeting BMP-2 transcript in fish

2014-02-01Journal article Restricted access
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dc.contributor.authorTiago, Daniel
dc.contributor.authorMarques, C. L.
dc.contributor.authorRoberto, Vania Palma
dc.contributor.authorCancela, Leonor
dc.contributor.authorLaizé, Vincent
dc.date.accessioned2014-06-06T16:23:10Z
dc.date.available2014-06-06T16:23:10Z
dc.date.issued2014-02-01
dc.date.updated2014-06-03T09:32:35Z
dc.description.abstractMicroRNAs (miRNAs) are important regulators of vertebrate development but their role during skeletogenesis remains unknown. In this regard, we investigated the mineralogenic activity of miR-20a, a miRNA associated with osteogenesis, in fish bone-derived cells. Expression of miR-20a was up-regulated during differentiation and its overexpression inhibited mineralization, suggesting a role in fish tissue calcification. In this regard, a conserved miR-20a binding site was identified in bone morphogenetic protein 2 (BMP-2) 30UTR and its functionality was evidenced through luciferase assays, and further confirmed by western-blot and qPCR. Type II BMP receptor (BMPR2) is also targeted by miR-20a in mammalian systems and evidence was collected for the presence of a binding site in fish sequences. We propose that miR-20a is a regulator of BMP pathway through specific action on BMP-2 and possibly BMPR2. Overexpression of miR-20a was also shown to up-regulate matrix Gla protein (MGP) transcript, a physiological inhibitor of calcification previously found to form a complex with BMP-2. We propose that MGP may play a role in the anti-mineralogenic effect promoted by miR-20a by decreasing availability of BMP-2. This study gives new insights into miRNA-mediated regulation of BMP-2, and sheds light into the potential role of miR-20a as a regulator of skeletogenesis.por
dc.identifier.citationTiago, Daniel M; Marques, Cátia L.; Roberto, Vânia P.; Cancela, M. Leonor; Laizé, Vincent. Mir-20a regulates in vitro mineralization and BMP signaling pathway by targeting BMP-2 transcript in fish, Archives of Biochemistry and Biophysics, 543, 543, 23-30, 2014.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.abb.2013.12.009
dc.identifier.issn0003-9861
dc.identifier.otherAUT: LCA00739
dc.identifier.urihttp://hdl.handle.net/10400.1/4233
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://ac.els-cdn.com/S0003986113003834/1-s2.0-S0003986113003834-main.pdf?_tid=0fb3b834-ed96-11e3-b9a1-00000aab0f02&acdnat=1402071631_8618ff0106f724c991c7e21e9de151d0por
dc.subjectBMP-2por
dc.subjectMineralizing fish bone-derived cellspor
dc.subjectPost-transcriptional regulationpor
dc.subjectMGP-BMP-2 interactionpor
dc.titleMir-20a regulates in vitro mineralization and BMP signaling pathway by targeting BMP-2 transcript in fishpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage30por
oaire.citation.startPage23por
oaire.citation.titleArchives of Biochemistry and Biophysicspor
oaire.citation.volume543por
person.familyNameTiago
person.familyNameRoberto
person.familyNameCancela
person.familyNameLaizé
person.givenNameDaniel
person.givenNameVânia
person.givenNameM. Leonor
person.givenNameVincent
person.identifier1230353
person.identifier.ciencia-id681E-40B7-331F
person.identifier.orcid0000-0001-8418-6292
person.identifier.orcid0000-0002-8134-4045
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0001-9565-9198
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id14422711000
person.identifier.scopus-author-id27467657800
person.identifier.scopus-author-id6602982778
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication10cda3a0-7b9c-4d16-bd2a-a06df1d56d94
relation.isAuthorOfPublicationc730a868-0e9f-43e4-a354-10130b30b764
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relation.isAuthorOfPublication.latestForDiscoveryc730a868-0e9f-43e4-a354-10130b30b764

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