Repository logo
 
Publication

Electrochemical noise and impedance of Au electrode/electrolyte interfaces enabling extracellular detection of glioma cell populations

2016-10Journal article Open Access
Simple item page
dc.contributor.authorSchlett, Paul
dc.contributor.authorKintzel, Ulrike
dc.contributor.authorMailaender, Volker
dc.contributor.authorVandamme, Lode K. J.
dc.contributor.authorZeck, Gunther
dc.contributor.authorGomes, Henrique L.
dc.contributor.authorBiscarini, Fabio
dc.contributor.authorde Leeuw, Dago M.
dc.date.accessioned2017-04-07T15:55:53Z
dc.date.available2017-04-07T15:55:53Z
dc.date.issued2016-10
dc.description.abstractMicroelectrode arrays (MEA) record extracellular local field potentials of cells adhered to the electrodes. A disadvantage is the limited signal-to-noise ratio. The state-of-the-art background noise level is about 10 mu Vpp. Furthermore, in MEAs low frequency events are filtered out. Here, we quantitatively analyze Au electrode/electrolyte interfaces with impedance spectroscopy and noise measurements. The equivalent circuit is the charge transfer resistance in parallel with a constant phase element that describes the double layer capacitance, in series with a spreading resistance. This equivalent circuit leads to a Maxwell-Wagner relaxation frequency, the value of which is determined as a function of electrode area and molarity of an aqueous KCl electrolyte solution. The electrochemical voltage and current noise is measured as a function of electrode area and frequency and follow unambiguously from the measured impedance. By using large area electrodes the noise floor can be as low as 0.3 mu Vpp. The resulting high sensitivity is demonstrated by the extracellular detection of C6 glioma cell populations. Their minute electrical activity can be clearly detected at a frequency below about 10 Hz, which shows that the methodology can be used to monitor slow cooperative biological signals in cell populations.
dc.identifier.doi10.1038/srep34843
dc.identifier.issn2045-2322
dc.identifier.otherAUT: HGO00803;
dc.identifier.urihttp://hdl.handle.net/10400.1/9247
dc.language.isoeng
dc.peerreviewedyes
dc.publisherNature Publishing Group
dc.relationImplantable Organic Devices for Advanced Therapies (INNOVATE) PTDC/EEI-AUT/5442/2014
dc.relationIntelligent Cell Surfaces (ICS)
dc.relation.isbasedonWOS:000384762600001
dc.titleElectrochemical noise and impedance of Au electrode/electrolyte interfaces enabling extracellular detection of glioma cell populations
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleIntelligent Cell Surfaces (ICS)
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EuroBioSAS%2F0001%2F2010/PT
oaire.citation.endPage34843
oaire.citation.startPage34843
oaire.citation.titleScientific Reports
oaire.citation.volume6
oaire.fundingStream3599-PPCDT
person.familyNameGomes
person.givenNameHenrique Leonel
person.identifier.orcid0000-0003-3664-4740
person.identifier.scopus-author-id7005305880
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication6da677b9-927f-423d-8657-448a0dccb67c
relation.isAuthorOfPublication.latestForDiscovery6da677b9-927f-423d-8657-448a0dccb67c
relation.isProjectOfPublicationf9ec75a2-1c41-437d-aee5-9a67dddb2591
relation.isProjectOfPublication.latestForDiscoveryf9ec75a2-1c41-437d-aee5-9a67dddb2591

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
9247.pdf
Size:
1.22 MB
Format:
Adobe Portable Document Format
Download

Collections

FCT2-Artigos (em revistas ou actas indexadas)