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Testis transcriptomic analyses reveal the effects of an algae feed on sperm quality in senegalese sole during the breeding season

dc.contributor.authorFélix de Azeredo Pinto e Melo, Francisca
dc.contributor.authorRaposo de Magalhães, Cláudia
dc.contributor.authorMarrero Alemán, Carlos
dc.contributor.authorDuarte, Daniel Filipe Correia
dc.contributor.authorParente, Pedro
dc.contributor.authorFatsini Fernández, Elvira
dc.contributor.authorOliveira, Catarina
dc.contributor.authorCabrita, Elsa
dc.date.accessioned2025-01-04T10:39:57Z
dc.date.available2025-01-04T10:39:57Z
dc.date.issued2025-03
dc.description.abstractThe usage of dietary algae antioxidants to improve fish reproduction is under-explored, especially in terms of the male reproductive system. In this experiment, 6 % of a blended meal of Phaeodactylum tricornutum and Gracilaria gracilis was incorporated in Senegalese sole broodstock feed, to evaluate the effects on sperm quality of F1 males throughout the breeding season. For that, two groups of breeders were fed during 6 months with the control and algae diets (6 % of control wheat meal replaced with 6 % algae blend). Every 2 weeks, fish were sampled for sperm quality evaluation, which included spermatozoa motility (CASA system), lipid peroxidation (MDA quantification), cell viability, reactive oxygen species (ROS), apoptotic status (flow cytometer), and DNA fragmentation (Comet assay). On a final sampling, 6 fish per group were sacrificed to dissect gonadal tissue, extract RNA and perform an RNA sequencing (RNA-seq) for each treatment. Sperm quality variability was high during the breeding season, including within the same month, irrespective of the diet. Cell viability was approximately 80 % during the whole experiment. Nonetheless, in specific sampling points, algae-fed fish showed higher spermatozoa protection against oxidative processes: in the 1st sampling live cells without ROS (%) were 3 times higher than in control group; on the last two samplings, spermatozoa showed half of MDA content; and on the 3rd sampling had less DNA fragmentation. No differences were found regarding apoptotic status. At the end of the reproductive season, gonadal transcriptomic analysis revealed that algae-fed fish were lacking stimuli for sperm production, both in terms of quantity and quality. This fish group seemed to have lipid metabolism and antioxidant capacity enhanced by the diet but, at the same time, were facing a compensatory mechanism due to an unknown algae compound that might be disrupting DNA replication and spermatogenesis. Altogether, this study suggests that algae blends can be used in broodstock feeds for Senegalese sole, however further research is needed to understand how to use only the desirable bioactive compounds and thus obtain higher and consistent sperm quality throughout the breeding season.eng
dc.identifier.doi10.1016/j.aquaculture.2024.741955
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10400.1/26577
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationImprovement of Senegalese sole sperm quality through the action of melatonin as an antioxidant
dc.relationMitigation solutions for the reproductive disorder of Senegalese sole cultivation – MITIGASOLE
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofAquaculture
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBroodstock nutrition
dc.subjectAntioxidants
dc.subjectGracilaria gracilis
dc.subjectPhaeodactylum tricornutum
dc.subjectSpermatogenesis
dc.subjectRNA-seq
dc.titleTestis transcriptomic analyses reveal the effects of an algae feed on sperm quality in senegalese sole during the breeding seasonpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleImprovement of Senegalese sole sperm quality through the action of melatonin as an antioxidant
oaire.awardTitleMitigation solutions for the reproductive disorder of Senegalese sole cultivation – MITIGASOLE
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F148280%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 3ed/2020.04181.CEECIND%2FCP1597%2FCT0002/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.startPage741955
oaire.citation.titleAquaculture
oaire.citation.volume598
oaire.fundingStreamCEEC IND 3ed
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameFélix de Azeredo Pinto e Melo
person.familyNameRaposo de Magalhães
person.familyNameMarrero Alemán
person.familyNameDuarte
person.familyNameParente
person.familyNameFatsini Fernández
person.familyNameOliveira
person.familyNameCabrita
person.givenNameFrancisca
person.givenNameCláudia
person.givenNameCarlos
person.givenNameDaniel Filipe Correia
person.givenNamePedro
person.givenNameElvira
person.givenNameCatarina
person.givenNameElsa
person.identifierhttps://scholar.google.es/citations?hl=en&pli=1&user=55mbsZIAAAAJ
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person.identifier.ridE-8523-2012
person.identifier.scopus-author-id35433190500
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