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Chrysotila pseudoroscoffensis as a source of high-value polar lipids with antioxidant activity: A lipidomic approach

2022Journal article Restricted access
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dc.contributor.authorMoreira, Ana S. P.
dc.contributor.authorGonçalves, Joana
dc.contributor.authorConde, Tiago A.
dc.contributor.authorCouto, Daniela
dc.contributor.authorMelo, Tânia
dc.contributor.authorMaia, Inês Beatriz
dc.contributor.authorPereira, Hugo
dc.contributor.authorSilva, Joana
dc.contributor.authorDomingues, M. Rosário
dc.contributor.authorNunes, Cláudia
dc.date.accessioned2022-11-30T10:48:32Z
dc.date.available2022-11-30T10:48:32Z
dc.date.issued2022
dc.description.abstractMicroalgae are emerging as sustainable sources of a wide range of high-value compounds. However, the knowledge about microalgae polar lipids is still limited, despite their interest due to their chemical diversity and bioactivity. This study shows, for the first time, the polar lipidome of the haptophyte microalga Chrysotila pseudoroscoffensis unveiled by using hydrophilic interaction liquid chromatography-high resolution mass spectrometry (HILIC-HRMS and MS/MS) and gas chromatography-mass spectrometry (GC-MS). Freeze-dried C. pseudoroscoffensis biomass has a lipid content of 6.4 %, containing higher amounts of ash (45.5 %), proteins (11.6 %), and sugars (11.0 %). Uronic acids (53.8 mol%) are the sugars present in higher content, followed by glucose (13.7 mol%) and galactose (12.7 mol%). The polar lipid species identified by HILIC-MS/MS included betaine lipids, glycolipids, and phospholipids, some of them with recognised bioactive properties. Among the lipid classes found from C. pseudoroscoffensis, some are less reported in algae: betaine lipids diacylglycerylcarboxyhydroxymethylcholine (DGCC) and monoacylglycerylcarboxyhydroxymethylcholine (MGCC) (characteristic of haptophyte microalgae); acid glycolipid class glucuronosyldiacylglycerol (GlcADG) (mainly reported in plants with protective effects in phosphate-deprivation conditions); and phospholipid classes monomethylphosphatidylethanolamine (MMPE) and lysomonomethylphosphatidylethanolamine (MMLPE). As estimated by colorimetric assays, glycolipids and phospholipids accounted for 64 and 3 % of the total lipid extracts, respectively. Fatty acid profiling by GC-MS showed that total esterified fatty acids accounted for about 32 % of the total lipid extracts, of which 23 % were omega-3 polyunsaturated fatty acids (PUFA). Four lipid extract concentrations (12.5, 62.5, 125 and 250 mu g mL-1 in ethanol) were tested and displayed antioxidant capacity toward 2,2 & PRIME;-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. A dose-dependent behaviour was observed with IC50 of 111.9 mu g mL(-1) for ABTS and IC35 of 234.8 mu g mL(-1) for DPPH assay. In conclusion, the lipid extracts of C. pseudoroscoffensis may be a source of high-value lipids for the development of novel microalgae-based products, namely nutraceuticals and cosmeceuticals.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.algal.2022.102756pt_PT
dc.identifier.issn2211-9264
dc.identifier.urihttp://hdl.handle.net/10400.1/18560
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.subjectAlgaept_PT
dc.subjectHaptophytespt_PT
dc.subjectCoccolithophorespt_PT
dc.subjectLipidomicspt_PT
dc.titleChrysotila pseudoroscoffensis as a source of high-value polar lipids with antioxidant activity: A lipidomic approachpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.citation.startPage102756pt_PT
oaire.citation.titleAlgal Researchpt_PT
oaire.citation.volume66pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMaia
person.familyNamePereira
person.givenNameInês Beatriz
person.givenNameHugo
person.identifier282781
person.identifier.ciencia-idE017-3A05-FF0A
person.identifier.ciencia-id331E-84FD-2F34
person.identifier.orcid0000-0002-7509-2216
person.identifier.orcid0000-0002-1369-2099
person.identifier.ridE-9360-2015
person.identifier.scopus-author-id54080259900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication7f541e4a-ff20-4a84-95d8-f0b915450330
relation.isAuthorOfPublicationebcb2180-5235-4502-b562-cd1e7786e6f9
relation.isAuthorOfPublication.latestForDiscoveryebcb2180-5235-4502-b562-cd1e7786e6f9
relation.isProjectOfPublicationfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f
relation.isProjectOfPublication.latestForDiscoveryfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f

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