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ISSR and AFLP characterization of Phomopsis amygdali ((Del.) Tuset & Portilla Accessions

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dc.contributor.authorCarlier, Jorge
dc.contributor.authorCabrita, L.
dc.contributor.authorLeitão, José
dc.contributor.authorSousa, R. M.
dc.contributor.authorSousa, A. T.
dc.date.accessioned2015-06-19T14:36:25Z
dc.date.available2015-06-19T14:36:25Z
dc.date.issued2011
dc.description.abstractThe fungus Phomopsis amygdali (Del.) Tuset & Portilla (sin: Fusicoccum amygdali Delacr.) is one of the most important almond (Prunus dulcis (Mill.) Webb) diseases in Portugal, causing cankers on one-year-old branches, and wilting and death of infected twigs and shoots. The disease leads frequently to tree decline and death. In the Algarve region of Portugal, the most common commercial cultivars show severe damages while some traditional almond cultivars seem not to be significantly affected. Infected plant material was collected from almond and peach trees. Fungi present in the infected tissues were isolated on PDA medium and transferred to the mycotec managed by the Estacao Nacional de Fruticultura Vieira Natividade (ENFVN). Some fungi accessions were identified as Eutypa lata (Pers.: Fr.) Tul. & C. Tul. Seventeen accessions, one identified as Eutypa and sixteen as Phomopsis, were analyzed by ISSR and AFLP techniques. Fourteen Phomospsis accessions, grouped in a main cluster, showed a coefficient of similarity (DICE) over 0.88. One of the putative Phomopsis accessions showed to be erroneously classified as it displays strong genetic similarity (DICE 0.92) to the Eutypa accession, while these two samples presented very low genetic similarity (DICE 0.20) to the main group of Phomopsis accessions. One sample, which we hypothesize to be of Cytospora sp. remained apart (DICE 0.15) from all the other accessions. The identification and cloning of molecular markers specific to each pathogenic species will be of major interest for further rapid identification of the causal agent of canker symptoms in almond.
dc.identifier.isbn978-90-66056-34-3
dc.identifier.issn0567-7572
dc.identifier.otherAUT: JLE00446
dc.identifier.urihttp://hdl.handle.net/10400.1/6498
dc.language.isoeng
dc.peerreviewedyes
dc.publisherInternational Society for Horticultural Science
dc.relation.isbasedonP-002-ZP3
dc.relation.ispartofActa Horticulturae
dc.titleISSR and AFLP characterization of Phomopsis amygdali ((Del.) Tuset & Portilla Accessions
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceSanliurfa, Turkey
oaire.citation.endPage651
oaire.citation.startPage645
oaire.citation.titleV International Symposium on Pistachios and Almonds
oaire.citation.title5th International Symposium on Pistachios and Almonds
oaire.citation.volume912
person.familyNameCarlier
person.familyNameLeitão
person.givenNameJorge
person.givenNameJosé
person.identifier258398
person.identifier.ciencia-id691D-A1BB-15CC
person.identifier.ciencia-id7A17-3E0A-73AD
person.identifier.orcid0000-0003-0675-2716
person.identifier.orcid0000-0003-4278-0415
person.identifier.ridAAH-3958-2020
person.identifier.scopus-author-id7102166949
person.identifier.scopus-author-id7005119796
rcaap.rightsrestrictedAccess
rcaap.typeconferenceObject
relation.isAuthorOfPublicationd4af2b00-61d6-4fd9-b05d-9ed235b5c7de
relation.isAuthorOfPublication0f990d1c-2913-4f8a-8fb6-f8b02721247f
relation.isAuthorOfPublication.latestForDiscovery0f990d1c-2913-4f8a-8fb6-f8b02721247f

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