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Focussed beam reflectance measurement (FBRM) monitoring of particle size and morphology in suspension cultures of Morinda citrifolia and Centaurea calcitrapa

2003Journal article Restricted access
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dc.contributor.authorJeffers, Paul
dc.contributor.authorRaposo, Sara
dc.contributor.authorLima-Costa, Maria Emília
dc.contributor.authorConnolly, Patricia
dc.contributor.authorGlennon, Brian
dc.contributor.authorKieran, Patricia
dc.date.accessioned2014-07-22T11:52:35Z
dc.date.available2014-07-22T11:52:35Z
dc.date.issued2003
dc.date.updated2014-07-22T10:20:35Z
dc.description.abstractLaser light scattering technology, as applied in the Lasentec focussed beam reflectance measurement (FBRM) system, was used to characterise two morphologically dissimilar plant cell suspension cultures, Morinda citrifolia and Centaurea calcitrapa. Shake-flask suspensions were analysed in terms of biomass concentration and aggregate size/shape over the course of typical batch growth cycles. For the heavily aggregated C. calcitrapa, biomass levels [from 10-160 g fresh weight (fw) l-1)] were linearly correlated with FBRM counts. For M. citrifolia, which grows in unbranched chains of 2-10 elongated cells, linear correlation of biomass concentration with FBRM counts was applicable in the range 0-100 g fw l-1; at higher levels (100-300 g fw l-1), biomass was non-linearly correlated with FBRM counts and length-weighted average FBRM chord length. For both cell systems, particle morphology (size/shape) was quantified using semi-automated digital image analysis. The average aggregate equivalent diameter (C. calcitrapa) and average chain length (M. citrifolia), determined using image analysis, closely tracked the FBRM average chord length. The data clearly demonstrate the potential for applying the FBRM technique for rapid characterisation of plant cell suspension cultures.por
dc.identifier.citationJeffers, Paul; Raposo, Sara; Lima-Costa, Maria-Emilia; Connolly, Patricia; Glennon, Brian; Kieran, Patricia M. Focussed beam reflectance measurement (FBRM) monitoring of particle size and morphology in suspension cultures of Morinda citrifolia and Centaurea calcitrapa , Biotechnology Letters, 25, 23, 2023-2028, 2003.por
dc.identifier.issn0141-5492
dc.identifier.otherAUT: SRA01180;
dc.identifier.urihttp://hdl.handle.net/10400.1/4791
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherKluwer Academic Publisherspor
dc.subjectCentaurea calcitrapapor
dc.subjectPlant cell culturepor
dc.subjectBiomass levelspor
dc.subjectFBRMpor
dc.subjectImage analysispor
dc.subjectMorphologypor
dc.titleFocussed beam reflectance measurement (FBRM) monitoring of particle size and morphology in suspension cultures of Morinda citrifolia and Centaurea calcitrapapor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2028por
oaire.citation.issue23por
oaire.citation.startPage2023por
oaire.citation.titleBiotechnology Letterspor
oaire.citation.volume25por
person.familyNameRaposo
person.familyNameLima Costa
person.givenNameSara
person.givenNameMaria Emília
person.identifier.ciencia-idF010-54F2-9E30
person.identifier.orcid0000-0001-5344-7349
person.identifier.orcid0000-0002-4433-5756
person.identifier.ridL-3673-2013
person.identifier.scopus-author-id7801639810
person.identifier.scopus-author-id7003633118
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication035b3010-5f47-4700-b196-402970ff2a69
relation.isAuthorOfPublication6eb9b8a6-dd51-4ace-809b-7d7de9cdfe36
relation.isAuthorOfPublication.latestForDiscovery6eb9b8a6-dd51-4ace-809b-7d7de9cdfe36

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