Repository logo
 
Publication

Hybrid nanosystems based on natural polymers as protein carriers for respiratory delivery: stability and toxicological evaluation

2015-06Journal article Open Access
Simple item page
dc.contributor.authorRodrigues, Susana
dc.contributor.authorCordeiro, Clara
dc.contributor.authorSeijo, Begona
dc.contributor.authorRemunan-Lopez, Carmen
dc.contributor.authorGrenha, Ana
dc.date.accessioned2018-12-07T14:53:29Z
dc.date.available2018-12-07T14:53:29Z
dc.date.issued2015-06
dc.description.abstractChitosan/carrageenan/tripolyphosphate nanoparticles were previously presented as holding potential for an application in transmucosal delivery of macromolecules, with tripolyphosphate demonstrating to contribute for both size reduction and stabilisation of the nanoparticles. This work was aimed at evaluating the capacity of the nanoparticles as protein carriers for pulmonary and nasal transmucosal delivery, further assessing their biocompatibility pattern regarding that application. Nanoparticles demonstrated stability in presence of lysozyme, while freeze-drying was shown to preserve their characteristics when glucose or sucrose were used as cryoprotectants. Bovine serum albumin was associated to the nanoparticles, which were successfully microencapsulated by spray-drying to meet the aerodynamic requirements inherent to pulmonary delivery. Finally, a satisfactory biocompatibility profile was demonstrated upon exposure of two respiratory cell lines (Calu-3 and A549 cells) to the carriers. A negligible effect on cell viability along with no alterations on transepithelial electrical resistance and no induction of inflammatory response were observed. (C) 2015 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipNational Portuguese funding through FCT - Fundacao para a Ciencia e a Tecnologia [PTDC/SAU-FCF/100291/2008, PTDC/DTP-FTO/0094/2012, PEst-OE/EQB/LA0023/2013]; Spanish Government (ISCIII, Accion Estrategica de Salud) [PS09/00816]; FCT [SFRH/BD/52426/2013]; ShareBiotech mobility grant; FCT project [PEst-OE/MAT/UI0006/2014]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.carbpol.2015.01.048
dc.identifier.issn0144-8617
dc.identifier.issn1879-1344
dc.identifier.urihttp://hdl.handle.net/10400.1/11539
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Sci Ltd
dc.relationFighting TB: Development of microparticulate systems to target alveolar macrophages in tuberculosis therapy
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMicroencapsulated chitosan nanoparticles
dc.subjectIn-vitro evaluation
dc.subjectDrug-delivery
dc.subjectCell-culture
dc.subjectChitosan/carrageenan nanoparticles
dc.subjectTransmucosal delivery
dc.subjectSilica nanoparticles
dc.subjectPulmonary delivery
dc.subjectChemical-stability
dc.subjectPlga nanoparticles
dc.titleHybrid nanosystems based on natural polymers as protein carriers for respiratory delivery: stability and toxicological evaluation
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleFighting TB: Development of microparticulate systems to target alveolar macrophages in tuberculosis therapy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0023%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FDTP-FTO%2F0094%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-FCF%2F100291%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FMAT%2FUI0006%2F2014/PT
oaire.citation.endPage380
oaire.citation.startPage369
oaire.citation.titleCarbohydrate Polymers
oaire.citation.volume123
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
person.familyNameRodrigues
person.familyNameHenrique Cordeiro
person.familyNameGrenha
person.givenNameSusana
person.givenNameClara Maria
person.givenNameAna
person.identifier.ciencia-id9D1A-6537-0383
person.identifier.ciencia-idC71E-21A1-E882
person.identifier.ciencia-id091C-0D58-7225
person.identifier.orcid0000-0002-0329-4265
person.identifier.orcid0000-0002-1026-6078
person.identifier.orcid0000-0002-2136-1396
person.identifier.ridH-1392-2017
person.identifier.scopus-author-id55125292100
person.identifier.scopus-author-id8607930100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublicationf7e806c8-708d-4caf-9b95-cfe9f3b06e8e
relation.isAuthorOfPublication7e0bbaff-c584-461a-84e2-54f2a6c15876
relation.isAuthorOfPublication1bd4d8f1-40e5-45af-b1e8-4b8e73a6a70d
relation.isAuthorOfPublication.latestForDiscovery7e0bbaff-c584-461a-84e2-54f2a6c15876
relation.isProjectOfPublication89fcacba-95e1-424f-aee5-1a0bdc3d7303
relation.isProjectOfPublication0d670626-9c73-4df8-b1ce-45987ba99ed5
relation.isProjectOfPublicationf5ee4293-e41f-48b6-adce-18b4d48260f7
relation.isProjectOfPublication2d773020-e58e-49c1-9089-6b2864d88f4a
relation.isProjectOfPublication.latestForDiscovery89fcacba-95e1-424f-aee5-1a0bdc3d7303

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
handle11539.pdf
Size:
2.07 MB
Format:
Adobe Portable Document Format
Download

Collections

FCT2-Artigos (em revistas ou actas indexadas)