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DNA cleavage activity of VIVO(acac)2 and derivatives

2009Journal article Restricted
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dc.contributor.authorButenko, Nataliya
dc.contributor.authorTomaz, Ana Isabel
dc.contributor.authorNouri, Ofelia
dc.contributor.authorEscribano, Esther
dc.contributor.authorMoreno, Virtudes
dc.contributor.authorGama, Sofia
dc.contributor.authorRibeiro, Vera
dc.contributor.authorTelo, João Paulo
dc.contributor.authorPessoa, João Costa
dc.contributor.authorCavaco, Isabel Maria Palma Antunes
dc.date.accessioned2013-12-18T10:00:58Z
dc.date.available2013-12-18T10:00:58Z
dc.date.issued2009
dc.date.updated2013-12-17T19:31:00Z
dc.description.abstractThe DNA cleavage activity of several b-diketonate vanadyl complexes is examined. Vanadyl acetylacetonate,VIVO(acac)2, 1, shows a remarkable activity in degrading plasmid DNA in the absence of any activating agents, air and photoirradiation. The cleaving activity of several related complexes VIVO(hd)2(2, Hhd = 3,5-heptanedione), VIVO(acac-NH2)2 (3, Hacac-NH2 = acetoacetamide) and VIVO(acac-NMe2)2(4, Hacac-NMe2 = N,N-dimethylacetoacetamide) is also evaluated. It is shown that 2 exhibits an activity similar to 1, while 3 and 4 are much less efficient cleaving agents. The different activity of the complexes is related to their stability towards hydrolysis in aqueous solution, which follows the order 1 2 3 4.The nature of the pH buffer was also found to be determinant in the nuclease activity of 1 and 2. In a phosphate buffered medium DNA cleavage by these agents is much more efficient than in tris, hepes,mes or mops buffers. The reaction seems to take place through a mixed mechanism, involving the formation of reactive oxygen species (ROS), namely OH radicals, and possibly also direct cleavage at phosphodiester linkages induced by the vanadium complexes.por
dc.identifier.citationButenko, Nataliya; Tomaz, Ana Isabel; Nouri, Ofelia; Escribano, Esther; Moreno, Virtudes; Gama, Sofia; Ribeiro, Vera; Telo, João Paulo; Pesssoa, João Costa; Cavaco, Isabel. DNA cleavage activity of VIVO(acac)2 and derivatives, Journal of Inorganic Biochemistry, 103, 4, 622-632, 2009.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.jinorgbio.2009.01.003
dc.identifier.issn0162-0134
dc.identifier.otherAUT: ICA01373;
dc.identifier.urihttp://hdl.handle.net/10400.1/3261
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectInorganic nucleasespor
dc.subjectDNA cleavagepor
dc.subjectVanadyl acetylacetonatepor
dc.subjectVanadium complexespor
dc.titleDNA cleavage activity of VIVO(acac)2 and derivativespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage632por
oaire.citation.issue4por
oaire.citation.startPage622por
oaire.citation.titleJournal of Inorganic Biochemistrypor
oaire.citation.volume103por
person.familyNameNatalia
person.familyNameRibeiro
person.givenNameButenko
person.givenNameVera
person.identifier.ciencia-id3411-732A-B7FB
person.identifier.orcid0000-0002-0214-8987
person.identifier.orcid0000-0002-9697-7830
person.identifier.ridC-2152-2018
person.identifier.ridG-3783-2011
person.identifier.scopus-author-id7006349364
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationa04db523-c55d-4c9c-93a7-c62a20bfe4c7
relation.isAuthorOfPublication3f188970-86bb-4815-afd8-e5e70f922fd9
relation.isAuthorOfPublication.latestForDiscoverya04db523-c55d-4c9c-93a7-c62a20bfe4c7

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