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Publication
In vitro propagation of insectivorous plants for phytochemical purposes
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Advisor(s)
Abstract(s)
The aims of this research work were to establish in vitro propagation protocols
for the insectivorous plants D. intermedia, D. rotundifolia and P. vulgaris, using
seeds as explants, in order to produce plants for biocompound extraction and to
replenish natural populations. The antioxidant capacity of the extracts prepared from
in vitro cultures of these species was evaluated.
The establishment of a simple protocol for in vitro propagation of D. intermedia
is reported. Protocols for micropropagation of P. vulgaris and D. rotundifolia were
initiated but still need to be improved. Cultures of all three species were initiated
from shoots of in vitro produced seedlings. The effect of three MS medium
concentrations, ¼MS, ½MS and MS, without PGR were tested in D. intermedia and
P. vulgaris. The same media supplemented with 0.1 mg l-1 KIN were investigated in
D. intermedia and ¼MS supplemented with 0.5 mg l-1 KIN or ZEA were
investigated in P. vulgaris. In all media D. intermedia shoots rooted during the
proliferation phase, presenting a multiplication and rooting percentage above 90%.
The highest mean number of shoots was attained in ¼MS and ¼MS supplemented
with 0.1 mg l-1 KIN. All the D. intermedia plants were successfully acclimatized to
ex vitro conditions. The formation of roots also occurred during P. vulgaris
proliferation phase, though with poorer results for both proliferation and rooting.
Nevertheless, the best results were obtained in the media supplemented with
cytokinins. In D. rotundifolia the media tested were ¼MS and MS. Multiplication
percentage and mean number of shoots was higher in ¼MS medium and no rooting
was observed.
Antioxidant activity of D. intermedia, D. rotundifolia and P. lusitanica extracts
was measured using three different methods: ORAC, F-C phenolics and TEAC.
Methanol and standard maceration improved the efficiency of the antioxidant
extraction procedure. For all tested methods P. lusitanica presented the highest
antioxidant activity, representing a possible good source of antioxidants.
Description
Dissertação mest., Engenharia Biológica, Universidade do Algarve, 2009
Keywords
Teses Bioquímica Biotecnologia Plantas insectívoras Pinguicula lusitanica Drosera