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In vitro metabolism of progesterone, androgens and estrogens by rainbow trout embryos

2002Journal article Restricted access
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dc.contributor.authorPetkam, R.
dc.contributor.authorRenaud, R. L.
dc.contributor.authorFreitas, A. M. M. S.
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorLeatherland, J. F.
dc.date.accessioned2014-05-15T17:45:33Z
dc.date.available2014-05-15T17:45:33Z
dc.date.issued2002
dc.date.updated2014-05-13T10:23:29Z
dc.description.abstractThe purpose of the study was to determine the steroid metabolic pathways used by rainbow trout (Oncorhynchus mykiss) embryos. Whole embryo preparations at 42, 53 and 65 days post fertilization (dpf) were incubated, in vitro, with the tritium-labelled steroids, progesterone (P4), testosterone (T), androstenedione (A4), 17β-estradiol (E2), and estrone (E1), and the metabolites formed were separated and identified by reverse phase high performance liquid chromatography (HPLC). The degree of metabolism for each of the substrates was dependent on the age of the embryos, and was always higher in older embryos. P4 yielded three metabolites, one of them identified by gas chromatography-mass spectroscopy as 3β,7α-dihydroxy-5α-pregnan-20-one. Several unknown metabolites of a similar profile on HPLC were produced for T and A4, in addition to A4 and T as metabolites of T and A 4 metabolism, respectively. Likewise, E2 and E1 yielded each other and an unknown polar metabolite. These observations argue for the presence of 5α-reductase, 7α-hydroxylase and 17β-hydroxysteroid dehydrogenase activity in embryo tissues which, by their conversion of biologically active yolk steroids of maternal origin, may provide a protective environment for the embryo during early developmentpor
dc.identifier.citationPetkam, R.; Renaud, R. L.; Freitas, A. M. M. S.; Canario, A. V. M.; Leatherland, J. F.In vitro metabolism of progesterone, androgens and estrogens by rainbow trout embryos, Fish Physiology and Biochemistry, 27, 1-2, 117-128, 2002.por
dc.identifier.doihttp://dx.doi.org/10.1023/B:FISH.0000021867.71729.22
dc.identifier.issn0920-1742
dc.identifier.otherAUT: ACA00258; AFR00371;
dc.identifier.urihttp://hdl.handle.net/10400.1/3980
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringer Verlag. Kluwer Academic Publisherspor
dc.relation.publisherversionhttp://link.springer.com/article/10.1023%2FB%3AFISH.0000021867.71729.22por
dc.subjectSteroidspor
dc.subjectHigh performance liquid chromatographypor
dc.subjectGas chromatography- mass spectroscopypor
dc.subjectProgesteronepor
dc.subjectAndrogenspor
dc.subjectEstrogenspor
dc.subjectSteroid metabolismpor
dc.subjectTeleost fishespor
dc.subjectEmbryospor
dc.titleIn vitro metabolism of progesterone, androgens and estrogens by rainbow trout embryospor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage128por
oaire.citation.issue1-2
oaire.citation.startPage117por
oaire.citation.titleFish Physiology and Biochemistrypor
oaire.citation.volume27por
person.familyNameCanario
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521

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