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Metatranscriptomes reveal functional variation in diatom communities from the Antarctic Peninsula

2015-10Journal article Open access
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dc.contributor.authorPearson, Gareth
dc.contributor.authorLago-Lestón, Asunción
dc.contributor.authorCanovas, Fernando
dc.contributor.authorCox, C. J.
dc.contributor.authorVerret, Frédéric
dc.contributor.authorLasternas, Sebastian
dc.contributor.authorDuarte, Carlos M.
dc.contributor.authorAgusti, Susana
dc.contributor.authorSerrao, Ester A.
dc.date.accessioned2018-12-07T14:58:04Z
dc.date.available2018-12-07T14:58:04Z
dc.date.issued2015-10
dc.description.abstractFunctional genomics of diatom-dominated communities from the Antarctic Peninsula was studied using comparative metatranscriptomics. Samples obtained from diatom-rich communities in the Bransfield Strait, the western Weddell Sea and sea ice in the Bellingshausen Sea/Wilkins Ice Shelf yielded more than 500K pyrosequencing reads that were combined to produce a global metatranscriptome assembly. Multi-gene phylogenies recovered three distinct communities, and diatom-assigned contigs further indicated little read-sharing between communities, validating an assembly-based annotation and analysis approach. Although functional analysis recovered a core of abundant shared annotations that were expressed across the three diatom communities, over 40% of annotations (but accounting for <10% of sequences) were community-specific. The two pelagic communities differed in their expression of N-metabolism and acquisition genes, which was almost absent in post-bloom conditions in the Weddell Sea community, while enrichment of transporters for ammonia and urea in Bransfield Strait diatoms suggests a physiological stance towards acquisition of reduced N-sources. The depletion of carbohydrate and energy metabolism pathways in sea ice relative to pelagic communities, together with increased light energy dissipation (via LHCSR proteins), photorespiration, and NO3 uptake and utilization all pointed to irradiance stress and/or inorganic carbon limitation within sea ice. Ice-binding proteins and cold-shock transcription factors were also enriched in sea ice diatoms. Surprisingly, the abundance of gene transcripts for the translational machinery tracked decreasing environmental temperature across only a 4 degrees C range, possibly reflecting constraints on translational efficiency and protein production in cold environments.
dc.description.sponsorshipPortuguese Science Foundation (FCT) [PTDC/MAR/72630]; Spanish Ministry of Economy and Competitiveness (under the scope of the International Polar Year (IPY)) [POL2006-00550/CTM]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1038/ismej.2015.40
dc.identifier.issn1751-7362
dc.identifier.issn1751-7370
dc.identifier.urihttp://hdl.handle.net/10400.1/11838
dc.language.isoeng
dc.peerreviewedyes
dc.publisherInternational Society for Microbial Ecology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectThalassiosira-pseudonana bacillariophyceae
dc.subjectPhotosynthetic energy-conversion
dc.subjectSea-ice diatoms
dc.subjectPhaeodactylum-tricornutum
dc.subjectSouthern-ocean
dc.subjectPhytoplankton biomass
dc.subjectMarine-phytoplankton
dc.subjectPelagic ecosystems
dc.subjectExtreme conditions
dc.subjectProtein-synthesis
dc.titleMetatranscriptomes reveal functional variation in diatom communities from the Antarctic Peninsula
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2289
oaire.citation.issue10
oaire.citation.startPage2275
oaire.citation.titleISME Journal
oaire.citation.volume9
person.familyNamePearson
person.familyNameLago-Lestón
person.familyNameCanovas
person.familyNameCox
person.familyNameSerrao
person.givenNameGareth Anthony
person.givenNameAsunción
person.givenNameFernando
person.givenNameCymon
person.givenNameEster A.
person.identifier113536
person.identifierC-6686-2012
person.identifier.ciencia-id3315-9919-1A52
person.identifier.ciencia-id6B15-9771-1D04
person.identifier.ciencia-id5B13-B26E-B1EC
person.identifier.orcid0000-0002-0768-464X
person.identifier.orcid0000-0003-4361-7732
person.identifier.orcid0000-0002-8837-1927
person.identifier.orcid0000-0002-4927-979X
person.identifier.orcid0000-0003-1316-658X
person.identifier.ridJ-3911-2013
person.identifier.ridM-3457-2013
person.identifier.ridD-1303-2012
person.identifier.scopus-author-id55916875600
person.identifier.scopus-author-id57193972525
person.identifier.scopus-author-id23395891600
person.identifier.scopus-author-id7402112716
person.identifier.scopus-author-id7004093604
rcaap.rightsopenAccess
rcaap.typearticle
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relation.isAuthorOfPublication.latestForDiscovery81132a0f-e241-4b49-8651-b989d1170775

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