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Exposure to silver and titanium dioxide nanoparticles at supra-environmental concentrations decreased sperm motility and affected spermatozoa subpopulations in gilthead seabream, Sparus aurata

2023-07Journal article Open Access
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dc.contributor.authorOliveira, Catarina
dc.contributor.authorFerrão, Leonor
dc.contributor.authorGallego, Victor
dc.contributor.authorMieiro, Cláudia
dc.contributor.authorOliveira, Isabel B.
dc.contributor.authorCarvalhais, Ana
dc.contributor.authorPachedo, Mário
dc.contributor.authorCabrita, Elsa
dc.date.accessioned2023-07-28T10:57:34Z
dc.date.available2023-07-28T10:57:34Z
dc.date.issued2023-07
dc.description.abstractMarine pollution by nanoparticles (NPs) can be reprotoxic for fish and disturb successful reproduction of wild populations. In gilthead seabream (Sparus aurata), a mild effect on sperm motility was observed after exposure to high concentrations of silver NPs. Considering the great heterogeneity traits within a sperm sample, it is possible that NPs affect spermatozoa accordingly, modulating subpopulation profile. Thus, this work aimed to analyse NP effects in sperm motility in general and considering spermatozoa population structure, using a subpopulation approach. Seabream sperm samples from mature males were exposed for 1 h to increasing concentrations of titanium dioxide (1, 10, 100, 1000 and 10,000 & mu;g L-1) and silver (0.25, 25 and 250 & mu;g L-1) NPs, including Ag NP and Ag+, dissolved in a non-activating medium (0.9 % NaCl). Concentrations chosen include realistic (10-100 and 0.25 & mu;g L-1, respectively, for TiO2 and Ag) and supra-environmental values. The mean particle diameter was determined as 19.34 & PLUSMN; 6.72 and 21.50 & PLUSMN; 8.27 nm in the stock suspension, respectively, for titanium dioxide and silver. After the ex vivo exposure, sperm motility parameters were determined using computer-assisted sperm analysis, and sperm subpopulations were later identified using a two-step cluster analysis. Results revealed a significant reduction in total motility after exposure to the 2 highest concentrations of titanium dioxide NPs, while curvilinear and straight-line velocities were not altered. Exposure to silver NPs (Ag NP and Ag+) lowered significantly total and progressive motilities at all concentrations, while curvilinear and straight-line velocities were significantly lower only at the highest concentration. Sperm subpopulations were also affected by the exposure to both titanium dioxide and silver NPs. In both cases, the highest levels of NPs triggered a decrease in the percentage of fast sperm subpopulations (38.2% in TiO2 1000 & mu;g L-1, 34.8.% in Ag NP 250 & mu;g L-1, and 45.0% in Ag+ 250 & mu;g L-1 vs 53.4% in the control), while an increase on slow sperm subpopulations. A reprotoxic effect was proven for both NPs, but only at supra-environmental concentrations.pt_PT
dc.description.sponsorshipOperational Program Competitiveness and Internationalization (POCI) POCI-01-0145-FEDER-30908, Fundacao para a Ciencia e a Tecnologia (FCT) SFRH/2020/05105/BD, LA/P/0101/2020, LA/P/0094/2020pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s10695-023-01218-0pt_PT
dc.identifier.eissn1573-5168
dc.identifier.urihttp://hdl.handle.net/10400.1/19890
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationUnraveling the ecological impacts of nanoparticles toxicity in the reproduction of marine organisms
dc.relationNot Available
dc.relationNot Available
dc.relationNot Available
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationGamete and embryo cryobanking for the preservation of threatened endemic fish
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectNanoparticlespt_PT
dc.subjectReprotoxicitypt_PT
dc.subjectEx vivo exposurept_PT
dc.subjectSperm subpopulationspt_PT
dc.titleExposure to silver and titanium dioxide nanoparticles at supra-environmental concentrations decreased sperm motility and affected spermatozoa subpopulations in gilthead seabream, Sparus auratapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleUnraveling the ecological impacts of nanoparticles toxicity in the reproduction of marine organisms
oaire.awardTitleNot Available
oaire.awardTitleNot Available
oaire.awardTitleNot Available
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleGamete and embryo cryobanking for the preservation of threatened endemic fish
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FCTA-AMB%2F30908%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL 57%2F2016%2FCP1361%2FCT0007/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL57%2F2016%2FCP1334%2FCP1644%2FCT0001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2018/CEECIND%2F01368%2F2018%2FCP1574%2FCT0005/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/101038049/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.citation.titleFish Physiology and Biochemistrypt_PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamDL 57/2016
oaire.fundingStreamDL 57/2016
oaire.fundingStreamCEEC IND 2018
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamH2020
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameOliveira
person.familyNameFerrão
person.familyNameGallego
person.familyNameCabrita
person.givenNameCatarina
person.givenNameLeonor
person.givenNameVictor
person.givenNameElsa
person.identifier.ciencia-id0816-30C5-51AC
person.identifier.ciencia-id8B13-2B8A-EDDE
person.identifier.orcid0000-0002-6679-761X
person.identifier.orcid0000-0001-9026-3522
person.identifier.orcid0000-0002-9733-0625
person.identifier.orcid0000-0003-3864-8841
person.identifier.ridK-9072-2017
person.identifier.ridE-8523-2012
person.identifier.scopus-author-id35433190500
person.identifier.scopus-author-id33367852200
person.identifier.scopus-author-id26645697000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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