Repository logo
 
Publication

Total DNA synthesis and cloning in Escherichia coli of a gene coding for the human growth hormone releasing factor

dc.contributor.authorCravador, A.
dc.contributor.authorJacobs, P.
dc.contributor.authorVan Elsen, A.
dc.contributor.authorLacroix, C.
dc.contributor.authorColau, B.
dc.contributor.authorVan Alphen, P.
dc.contributor.authorHerzog, A.
dc.contributor.authorBollen, A.
dc.date.accessioned2015-06-15T09:15:50Z
dc.date.available2015-06-15T09:15:50Z
dc.date.issued1985
dc.description.abstractA DNA containing a sequence coding for the human growth hormone releasing factor (hGRF) has been obtained by enzymatic assembly of chemically synthesized DNa fragments. The synthetic gene consists of a 140 base-pair fragment containing initiation and termination signals for translation and appropriate protruding ends for cloning into a newly constructed plasmid vector (pULB1219). Eleven oligodeoxyribonucleotides, from 14 to 31 bases in length, sharing pairwise stretches of complementary regions of at least 13 bases were prepared by phosphotriester solid-phase synthesis. The DNA sequence was designed to take into account the optimal use of E. coli codons. Oligomers were annealed in one step and assembled by ligation. The DNA fragment of the expected size (140 bp) was recovered and cloned into the pULB1219 vector. The expected sequence was confirmed by DNA sequencing. © 1985 Masson, Paris.
dc.identifier.issn0300-9084
dc.identifier.otherAUT: ACR00659;
dc.identifier.urihttp://hdl.handle.net/10400.1/6127
dc.language.isoeng
dc.peerreviewedyes
dc.relation.isbasedonP-008-R6K
dc.titleTotal DNA synthesis and cloning in Escherichia coli of a gene coding for the human growth hormone releasing factor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage834
oaire.citation.startPage829
oaire.citation.titleBiochimie
oaire.citation.volume67
person.familyNameCravador
person.givenNameAlfredo
person.identifier.orcid0000-0002-9831-9815
person.identifier.ridK-7247-2012
person.identifier.scopus-author-id6602537257
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication8c84a9e6-6b7f-4b50-93a2-152c85901722
relation.isAuthorOfPublication.latestForDiscovery8c84a9e6-6b7f-4b50-93a2-152c85901722

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Total DNA.pdf
Size:
1.76 MB
Format:
Adobe Portable Document Format