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High-glucose feeding of gilthead seabream (Sparus aurata) larvae: effects on molecular and metabolic pathways

2016-01Journal article Restricted access
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dc.contributor.authorRocha, Filipa
dc.contributor.authorDias, Jorge
dc.contributor.authorGeurden, Inge
dc.contributor.authorDinis, Maria Teresa
dc.contributor.authorPanserat, Stephane
dc.contributor.authorEngrola, S.
dc.date.accessioned2017-04-07T15:57:15Z
dc.date.available2017-04-07T15:57:15Z
dc.date.issued2016-01
dc.description.abstractNutritional programming has begun to arouse interest as a novel tool to alter specific metabolic pathways or functions in farmed animals. The aim of the present study was to explore the potential of early glucose stimuli to induce changes in nutrient metabolism of gilthead seabream. Nutritional conditioning was performed by delivering glucose-rich feed at three distinct recurrent periods of larval feeding regime: during first-feeding with rotifers (3 days after hatching, DAH) and mid-feeding with Artemia metanauplii (20DAH) and the beginning of inert diet feeding (30DAH), called the Recurrent treatment (REC). As opposed, the control treatment (CTRL) did not experience any glucose stimuli. At post-larval stage (from 50 to 60DAH), both treatments were challenged with a high-carbohydrate diet (50%). The immediate response to the early stimuli was assessed through gene expression of metabolic markers and by nutrient metabolism using [C-14] tracers. Each dietary stimulus induced metabolic changes on REC larvae, shown by altered expression of some genes, including those involved in glycolysis, and by a different pattern of glucose utilization. However, none of the molecular adaptations (except G6PDH gene) were persistent in the viscera and muscle of challenged post-larvae from REC group. In contrast, the glucose metabolism of challenged REC post-larvae revealed a shift towards a higher catabolism and lower glucose retention in tissues, compared to the CTRL group, suggesting an improvement of glucose oxidation pathways. In addition, the REC group showed a higher bio-conversion of glucose into lipids, indicating enhanced hepatic lipogenesis. The early stimuli did not affect the relative retention or use of amino acids or the growth and survival of challenged fish, up to 60DAH. In summary, although not substantiated at a molecular level, our data reveal that a recurrent high-glucose stimulus during larval stages affects the short-term modulation of pathways for glucose utilization in gilthead seabream. (C) 2015 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.aquaculture.2015.09.015
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10400.1/9657
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationEARLY NUTRITIONAL PROGRAMMING IN FISH: TAILORING THE METABOLIC USE OF DIETARY GLUCOSE
dc.relationAdvanced Research Initiatives for Nutrition & Aquaculture
dc.relation.isbasedonWOS:000364854800031
dc.subjectCarbohydrates
dc.subjectGlucose metabolism
dc.titleHigh-glucose feeding of gilthead seabream (Sparus aurata) larvae: effects on molecular and metabolic pathways
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEARLY NUTRITIONAL PROGRAMMING IN FISH: TAILORING THE METABOLIC USE OF DIETARY GLUCOSE
oaire.awardTitleAdvanced Research Initiatives for Nutrition & Aquaculture
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F74921%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F49051%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/288925/EU
oaire.citation.endPage253
oaire.citation.startPage241
oaire.citation.titleAquaculture
oaire.citation.volume451
oaire.fundingStreamSFRH
oaire.fundingStreamFP7
person.familyNameRocha
person.familyNameDinis
person.familyNameEngrola
person.givenNameFilipa
person.givenNameMaria Teresa
person.givenNameSofia
person.identifier237846
person.identifier.ciencia-id5414-4ACF-BF90
person.identifier.ciencia-idA610-29E7-9B83
person.identifier.orcid0000-0002-7531-9302
person.identifier.orcid0000-0001-7813-1324
person.identifier.orcid0000-0002-5244-5541
person.identifier.ridA-2485-2012
person.identifier.scopus-author-id7004150905
person.identifier.scopus-author-id8669620600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccess
rcaap.typearticle
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relation.isAuthorOfPublication309dec19-8aa9-4bea-8665-42dd4b3a9894
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