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Sex-biased miR-456 targeting spire1/sox11 provides insights into the ceRNA-network of sexual regulation in fish

2025-07Artigo científico Acesso aberto
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datacite.subject.sdg14:Proteger a Vida Marinha
datacite.subject.sdg02:Erradicar a Fome
datacite.subject.sdg04:Educação de Qualidade
dc.contributor.authorZhao, Na
dc.contributor.authorHe, Xiaoxu
dc.contributor.authorMin, Qianwen
dc.contributor.authorPower, Deborah Mary
dc.contributor.authorDong, Zhongdian
dc.contributor.authorYang, Changgeng
dc.contributor.authorZhang, Bo
dc.date.accessioned2026-04-10T08:45:54Z
dc.date.available2026-04-10T08:45:54Z
dc.date.issued2025-07
dc.description.abstractCompeting endogenous RNAs (ceRNAs) are a novel epigenetic regulatory mechanism implicated in sex determination and differentiation in teleosts, which exhibit a diversity of sex-determining mechanisms. In this study, based on whole transcriptome sequencing data, a ceRNA regulatory network composed of sex-inclined miRNAs (miR-456), lncRNAs (ASTR), circRNAs (circklhl29), and target mRNAs (spire1 and sox11) was uncovered in the gonadal tissues of Seriola dumerili, an oceanic species with lagged gonadal development. MiRNA-456 was identified as the core of the ceRNA regulatory network and suppressed the expression of spire1 and sox11, interacting with the ncRNAs including circklhl29 and ASTR. Co-localization of these sex-biased ncRNAs and mRNAs in the gonads suggests that this ceRNA network modulated the gonadal differentiation in both sexes of S. dumerili. In vivo injection in the gonads of S. dumerili suggested the miR-456 agonist could significantly inhibit expression of sox11 and spire1 in male testes, while significant regulatory effects of the miR-456 agonist and antagonist on sox11 and spire1 targets were not observed in female ovaries. The conserved binding sites for sequences of miR-456 and spire1/sox11 targets in various fish species were aligned and dual luciferase reporter gene experiments clarified the universality of the mechanism by which miR-456 bound to inhibit sox11/spire1 targets in large yellow croaker (Larimichthys crocea), half-smooth tongue sole (Cynoglossus semilaevis), and zebrafish (Danio rerio). These results further support the notion that ceRNA networks may be a universal regulatory system in teleosts despite their highly divergent sex regulation programs.eng
dc.description.sponsorshipZJW-2023-01; 2023A1515010576; 20241515012859; 2023E0006
dc.identifier.doi10.1016/j.watbs.2025.100370
dc.identifier.issn2772-7351
dc.identifier.urihttp://hdl.handle.net/10400.1/28635
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relation.ispartofWater Biology and Security
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectMiR-456
dc.subjectSpire1
dc.subjectSox11
dc.subjectcernas
dc.subjectSex
dc.subjectSeriola dumerili
dc.titleSex-biased miR-456 targeting spire1/sox11 provides insights into the ceRNA-network of sexual regulation in fisheng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue3
oaire.citation.startPage100370
oaire.citation.titleWater Biology and Security
oaire.citation.volume4
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamePower
person.givenNameDeborah Mary
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7101806760
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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