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SAMMBA is a high-throughput pipeline for isolating and phenotyping macroalgal strains

2026-03Artigo científico Acesso aberto
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datacite.subject.sdg14:Proteger a Vida Marinha
datacite.subject.sdg02:Erradicar a Fome
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
dc.contributor.authorAlves-Lima, Cicero
dc.contributor.authorde Matos Barreto, Luís António
dc.contributor.authorMonico, Carina
dc.contributor.authorGouvêa, Lidiane
dc.contributor.authorFélix de Azeredo Pinto e Melo, Francisca
dc.contributor.authorVarga, Brigitta
dc.contributor.authorFilipe, Joana
dc.contributor.authorCamacho, Rita
dc.contributor.authorLymperaki, Myrsini
dc.contributor.authorAlberto, Filipe
dc.contributor.authorRörig, Leonardo R.
dc.contributor.authorEngelen, Aschwin
dc.contributor.authorSerrao, Ester A.
dc.contributor.authorPearson, Gareth Anthony
dc.contributor.authorMartins, Neusa
dc.date.accessioned2026-03-20T17:46:31Z
dc.date.available2026-03-20T17:46:31Z
dc.date.issued2026-03
dc.description.abstractDespite successful preservation efforts, macroalgal diversity remains under-represented in global biobanks. A major limitation is the extreme morphological diversity of seaweed thalli, which hinders standardized isolation and phenotyping and often requires taxon-specific protocols. Here we present SAMMBA (Seaweed Automatable Microplate Microscopy for Breeding Approaches), an end-to-end pipeline for the high-throughput isolation, phenotyping and storage of macroalgal propagules in 384-well plates. By optimizing live-cell manipulation for chlorophyll autofluorescence (CAF) imaging and segmentation, multiple unialgal propagules can be isolated by dilution-based workflows. In a single plate, we obtained 68 singlet gametophyte fragments of Laminaria ochroleuca (17.7%) and 60 meiospores of Phyllariopsis purpurascens (31.25%). We demonstrated taxonomic and morphological versatility by isolating 60 unialgal cultures from three distinct Rhodophyta morphotypes (filamentous, crustose and foliose) and 10 strains of Ulva sp., also in a single plate. Furthermore, CAF-based area increase over 30 days enabled high-precision estimates of specific growth rates, yielding 0.130 ± 0.006 and 0.117 ± 0.01 day− 1 for male and female L. ochroleuca gametophytes, respectively (n = 768; p = 1.27e− 53). This precision substantially increases experimental reproducibility and statistical power compared to conventional methods, supporting high-throughput recovery of unialgal strains without motorized platforms, while remaining fully compatible with automation. SAMMBA expands operational capacity for strain discovery and phenotyping, providing a scalable foundation for phenomics, domestication workflows, and standardized macroalgal biobanking. We outline how the platform can benefit multiple areas of phycological research and facilitate the development of improved strains that can support aquaculture and restoration efforts.eng
dc.description.sponsorshipUID/04326/2025; UID/PRR/04326/2025
dc.identifier.doi10.1016/j.algal.2026.104547
dc.identifier.issn2211-9264
dc.identifier.urihttp://hdl.handle.net/10400.1/28499
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofAlgal Research
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectSeaweed phenomics
dc.subjectChlorophyll fluorescence
dc.subjectBreeding automation
dc.subjectMarine biotechnology
dc.subjectBioimaging
dc.titleSAMMBA is a high-throughput pipeline for isolating and phenotyping macroalgal strainseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberLA/P/0101/2020
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.startPage104547
oaire.citation.titleAlgal Research: Biomass, Biofuels and Bioproducts
oaire.citation.volume94
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameAlves-Lima
person.familyNamede Matos Barreto
person.familyNameMonico
person.familyNameGouvêa
person.familyNameFélix de Azeredo Pinto e Melo
person.familyNameVarga
person.familyNameFilipe
person.familyNameCamacho
person.familyNameLymperaki
person.familyNameEngelen
person.familyNameSerrao
person.familyNamePearson
person.familyNameMartins
person.givenNameCicero
person.givenNameLuís António
person.givenNameCarina
person.givenNameLidiane
person.givenNameFrancisca
person.givenNameBrigitta
person.givenNameJoana
person.givenNameRita
person.givenNameMyrsini
person.givenNameAschwin
person.givenNameEster A.
person.givenNameGareth Anthony
person.givenNameNeusa
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
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