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Drug resistance and epigenetic modulatory potential of epigallocatechin-3-gallate against staphylococcus aureus

2022-05Journal article Restricted access
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dc.contributor.authorZeferino, Ana Sofia
dc.contributor.authorMira, Ana Rita
dc.contributor.authorDelgadinho, Mariana
dc.contributor.authorBrito, Miguel
dc.contributor.authorPonte, Tomás
dc.contributor.authorRibeiro, Edna
dc.date.accessioned2022-09-22T10:04:57Z
dc.date.available2022-09-22T10:04:57Z
dc.date.issued2022-05
dc.description.abstractAntimicrobial resistance of human pathogens, such as methicillin-resistant Staphylococcus aureus, is described by the World Health Organization as a health global challenge and efforts must be made for the discovery of new effective and safe compounds. This work aims to evaluate epigallocatechin-3-gallate (EGCG) epigenetic and modulatory drug potential against S. aureus in vitro and in vivo. S. aureus strains were isolated from commensal flora of healthy volunteers. Antibiotic susceptibility and synergistic assay were assessed through disk diffusion accordingly to EUCAST guidelines with and without co-exposure to EGCG at final concentrations of 250 mu g/ml, 100 mu g/ml, 50 mu g/ml, and 25 mu g/ml. Transcriptional expression of orfx, spdC, and WalKR was performed through qRT-PCR. A 90-day interventional study was performed with daily consumption of 225 mg of EGCG. Obtained data revealed a high prevalence of S. aureus colonization in healthcare workers and clearly demonstrated the antimicrobial and synergistic potential of EGCG as well as divergent resistant phenotypes associated with altered transcriptional expression of epigenetic and drug response modulators genes. Here, we demonstrate the potential of EGCG for antimicrobial treatment and/or therapeutic adjuvant against antibiotic-resistant microorganisms and report divergent patterns of epigenetic modulators expression associated with phenotypic resistance profiles.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00284-022-02841-5pt_PT
dc.identifier.eissn1432-0991
dc.identifier.urihttp://hdl.handle.net/10400.1/18279
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationHealth and Technology Research Center
dc.relationHealth and Technology Research Center
dc.subjectEthicillin resistancept_PT
dc.subjectAntibiotic-resistancept_PT
dc.subjectNasal carriagept_PT
dc.subjectMEC SCCMECpt_PT
dc.subjectGallatept_PT
dc.subjectGenept_PT
dc.subjectMechenismspt_PT
dc.subjectComponentpt_PT
dc.subjectSynergypt_PT
dc.subjectSitept_PT
dc.titleDrug resistance and epigenetic modulatory potential of epigallocatechin-3-gallate against staphylococcus aureuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth and Technology Research Center
oaire.awardTitleHealth and Technology Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05608%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F05608%2F2020/PT
oaire.citation.issue5pt_PT
oaire.citation.titleCurrent Microbiologypt_PT
oaire.citation.volume79pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePonte
person.givenNameTomás
person.identifier.orcid0000-0002-9984-0610
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication652bf8fd-0680-42aa-bcb7-ad027d728814
relation.isAuthorOfPublication.latestForDiscovery652bf8fd-0680-42aa-bcb7-ad027d728814
relation.isProjectOfPublication19f919ca-4b5b-4d6c-92c7-958102c6fb97
relation.isProjectOfPublication48c45812-750b-4874-b594-92ef343c8e47
relation.isProjectOfPublication.latestForDiscovery19f919ca-4b5b-4d6c-92c7-958102c6fb97

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