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Distribution, identification and cytotoxicity of Gambierdiscus (Dinophyceae) in the Atlantic Selvagens Islands (Madeira, Portugal): a ciguatera gateway to Europe

2022-07Journal article Restricted access
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dc.contributor.authorGodinho, Lia
dc.contributor.authorSoliño, Lucia
dc.contributor.authorChurro, Catarina
dc.contributor.authorTimoteo, Viriato
dc.contributor.authorSantos, Carolina
dc.contributor.authorGouveia, Neide
dc.contributor.authorDiogène, Jorge
dc.contributor.authorReis Costa, Pedro
dc.date.accessioned2022-12-09T11:39:08Z
dc.date.available2022-12-09T11:39:08Z
dc.date.issued2022-07
dc.description.abstractThe emerging threat of ciguatera poisoning (CP) in Europe has been associated with fish captured in the Canary Islands (Spain) and Selvagens Islands (Portugal). The first are heavily populated islands where numerous scientific studies have been carried out. Conversely, the Selvagens Islands are a nature reserve with low human pressure that have been rarely surveyed in terms of the marine benthic microalgae, including the epiphytic ciguatera-causing dinoflagellate species. To investigate the harmful microalgal diversity of the Selvagens Islands, a scientific cruise to these remote islands took place in September, 2018. The Gambierdiscus species composition and distribution, and the associated epiphytic dinoflagellate community, were assessed using artificial substrate devices. Gambierdiscus cells were found in all samples, reaching concentrations of up to 725 cells 100 cm(-2). G. australes was the only species identified after morphological and molecular analysis of the retrieved cultures. Species identification was confirmed by molecular characterization based on the LSU D8-D10 region. Nevertheless, phylogenetic studies indicated that some strains diverged from the G. australes clade suggesting genetic differentiation. Toxicity was estimated by neuro-2a cell-based assay in four strains, ranging from 2.46-83 fg of CTX1B eq. cell(-1). The epiphytic dinoflagellate community that co-occurred with Gambierdiscus comprised other toxic or potentially toxic dinoflagellates, such as Ostreopsis, Prorocentrum, Amphidinium and Coolia species. Oceanographic and meteorological data were also obtained to characterize the occurrence of Gambierdiscus. This study is the first stage in understanding the role of the Selvagens Islands in the incubation and proliferation of the ciguatera-causing dinoflagellates Gambierdiscus in the NE Atlantic.pt_PT
dc.description.sponsorshipMar-02.01.01-FEAMP-0045; GP/EFSA/AFSCO/2015/03
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1080/09670262.2022.2086710pt_PT
dc.identifier.eissn1469-4433
dc.identifier.urihttp://hdl.handle.net/10400.1/18608
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.subjectAlexandriumpt_PT
dc.subjectBenthic dinoflagellatespt_PT
dc.subjectCiguaterapt_PT
dc.subjectCyanotoxinspt_PT
dc.subjectDinoflagellatept_PT
dc.subjectGambierdiscuspt_PT
dc.subjectLSU rDNApt_PT
dc.subjectMadeirapt_PT
dc.subjectSeafood safetypt_PT
dc.subjectSelvagens Islandspt_PT
dc.titleDistribution, identification and cytotoxicity of Gambierdiscus (Dinophyceae) in the Atlantic Selvagens Islands (Madeira, Portugal): a ciguatera gateway to Europept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FCTA-AMB%2F30557%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04423%2F2020/PT
oaire.citation.endPage13pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleEuropean Journal of Phycologypt_PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSoliño
person.familyNameReis Costa
person.givenNameLucia
person.givenNamePedro
person.identifier600820
person.identifier.ciencia-id2F12-68AC-8F6C
person.identifier.ciencia-idC911-9715-E547
person.identifier.orcid0000-0001-8356-5688
person.identifier.orcid0000-0001-6083-470X
person.identifier.ridN-1908-2019
person.identifier.scopus-author-id7201895802
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication27e1ebce-5931-49c1-9d91-53c69d8e255b
relation.isAuthorOfPublication24ad7119-d61f-4bdb-a5d5-9bd6e2c37312
relation.isAuthorOfPublication.latestForDiscovery27e1ebce-5931-49c1-9d91-53c69d8e255b
relation.isProjectOfPublication9225a542-fab2-426b-ad6c-12e24012212d
relation.isProjectOfPublication2918f082-1ced-47c5-a6ea-eff9b8eaca1f
relation.isProjectOfPublication.latestForDiscovery2918f082-1ced-47c5-a6ea-eff9b8eaca1f

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