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Culturable yeasts associated to different life stages of the farmed red seaweed Porphyra dioica

2026-08-12Artigo científico Acesso aberto
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datacite.subject.sdg14:Proteger a Vida Marinha
datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg02:Erradicar a Fome
dc.contributor.authorFernandes, Mónica A.
dc.contributor.authorMatos, Madalena
dc.contributor.authorKeller-Costa, Tina
dc.contributor.authorCosta, Rodrigo
dc.contributor.authorCaria Mendes, Madalena
dc.contributor.authorCoutinho, Ana
dc.contributor.authorOliveira, Inês
dc.contributor.authorMartins, Margarida
dc.contributor.authorSá-Correia, Isabel
dc.date.accessioned2026-09-21T15:43:29Z
dc.date.available2026-09-21T15:43:29Z
dc.date.issued2026-08-12
dc.description.abstractMacroalgal-associated microbiomes are increasingly recognized as key determinants of host fitness, biogeochemical cycling, and cultivation stability in industrial aquaculture. While bacterial assemblages of red macroalgae have received considerable attention, the associated mycobiome, and in particular the yeast fraction, remains poorly characterized, despite mounting evidence that fungal taxa contribute to phytohormone signaling, surface colonization dynamics, and host stress tolerance. In this study, we characterized the culturable yeast fraction associated with five life stages of the red macroalga Porphyra dioica cultivated in an Integrated Multi-Trophic Aquaculture (IMTA) system in Ílhavo, Portugal. Yeast isolates were recovered from conchocelis, conchosporangia, young blade, and adult blade stages by direct plating and enrichment on selective media and taxonomically assigned by rRNA gene sequencing. Isolates were further screened for functional traits of ecological and biotechnological relevance, including auxin biosynthesis, biosurfactant and bioemulsifier production, and intracellular lipid accumulation. A total of 21 isolates were recovered and assigned to Rhodotorula mucilaginosa, Naganishia diffluens, Williopsis vartiovaarae, Taphrina sp., and Sporobolomyces roseus, spanning both Ascomycota and Basidiomycota. Yeast recovery was constrained by chloramphenicol-resistant bacterial overgrowth in algae samples cultivated with filtered, non-sterilized seawater, and therefore the dataset represents the recoverable culturable fraction. Assemblages were structured by host developmental stage: N. diffluens was recovered from conchocelis, R. mucilaginosa from conchosporangia, and a shift from ascomycetous taxa (W. vartiovaarae, Taphrina sp.) to pigmented basidiomycetous yeasts (N. diffluens, S. roseus) was observed in adult blades approaching harvest. Several isolates produced auxin, biosurfactants, and bioemulsifiers, and accumulated intracellular lipids, supporting potential roles as probiotics in industrial macroalgal cultivation and as candidate strains for value-added bioproducts. This study provides a first insight into the culturable yeast community associated with industrially cultivated P. dioica and establishes a basis for future studies addressing the role of yeasts in macroalgal performance cultivated under IMTA systems.eng
dc.description.sponsorshipUID/04565/2025
dc.identifier.doi10.3389/fmicb.2026.1918765
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10400.1/29491
dc.language.isoeng
dc.peerreviewedyes
dc.publisherFrontiers Media SA
dc.relationInstitute for Health and Bioeconomy
dc.relation.ispartofFrontiers in Microbiology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAlgal-associated yeasts
dc.subjectBiotechnological potential
dc.subjectCulturable marine yeasts
dc.subjectFarmed algae
dc.subjectIMTA system
dc.subjectLife stage distribution
dc.subjectYeast diversity
dc.titleCulturable yeasts associated to different life stages of the farmed red seaweed Porphyra dioicaeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberLA/P/0140/2020
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT
oaire.citation.titleFrontiers in Microbiology
oaire.citation.volume17
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCaria Mendes
person.givenNameMadalena
person.identifier.ciencia-id9C12-FB3A-81F7
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication2eab4c0e-9099-454d-8043-656a7710a381
relation.isAuthorOfPublication.latestForDiscovery2eab4c0e-9099-454d-8043-656a7710a381
relation.isProjectOfPublicationaa89733d-ccad-4fd3-b596-27382f53b0ca
relation.isProjectOfPublication.latestForDiscoveryaa89733d-ccad-4fd3-b596-27382f53b0ca

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