Logo do repositório
 
A carregar...
Miniatura
Publicação

Spindly and Bub3 expression in oral cancer: Prognostic and therapeutic implications

Utilize este identificador para referenciar este registo.
Nome:Descrição:Tamanho:Formato: 
odi.13089.pdf1.07 MBAdobe PDF Ver/Abrir

Orientador(es)

Resumo(s)

Objectives Bub3 and Spindly are essential proteins required for the activation and inactivation of the spindle assembly checkpoint, respectively. Here, we explored the clinicopathological significance and the therapeutic potential of the opposing roles of the two proteins in oral squamous cell carcinoma (OSCC). Materials and Methods Bub3 and Spindly expression was evaluated by immunohistochemistry in 62 tissue microarrays from OSCC and by real-time PCR in OSCC cell lines and in normal human oral keratinocytes. The results were analyzed as to their clinicopathological significance. RNA interference-mediated Spindly or Bub3 inhibition was combined with cisplatin treatment, and the effect on the viability of OSCC cells was assessed. Results Overexpression of Bub3 and Spindly was detected in OSCC patients. High expression of Spindly, Bub3, or both was an independent prognostic indicator for cancer-specific survival and was associated with increased cellular proliferation. Accordingly, Bub3 and Spindly were upregulated in OSCC cells comparatively to their normal counterpart. Inhibition of Bub3 or Spindly was cytotoxic to OSCC cells and enhanced their chemosensitivity to cisplatin. Conclusions The data point out Bub3 and Spindly as potential markers of proliferation and prognosis, and highlight the potential therapeutic benefit of combining their inhibition with cisplatin.

Descrição

Palavras-chave

Assembly checkpoint Chromosomal instability Dynein Cisplatin Kinetochores Complex Cells

Contexto Educativo

Citação

Projetos de investigação

Unidades organizacionais

Fascículo

Editora

Wiley

Licença CC

Métricas Alternativas