Repository logo
 
Publication

Spindly and Bub3 expression in oral cancer: Prognostic and therapeutic implications

2019-07Journal article Restricted access
Simple item page
dc.contributor.authorSilva, Patricia M. A.
dc.contributor.authorDelgado, Maria Leonor
dc.contributor.authorRibeiro, Nilza
dc.contributor.authorFlorindo, Claudia
dc.contributor.authorTavares, Alvaro A.
dc.contributor.authorRibeiro, Diana
dc.contributor.authorLopes, Carlos
dc.contributor.authordo Amaral, Barbas
dc.contributor.authorBousbaa, Hassan
dc.contributor.authorMonteiro, Luis Silva
dc.date.accessioned2020-07-24T10:52:04Z
dc.date.available2020-07-24T10:52:04Z
dc.date.issued2019-07
dc.description.abstractObjectives Bub3 and Spindly are essential proteins required for the activation and inactivation of the spindle assembly checkpoint, respectively. Here, we explored the clinicopathological significance and the therapeutic potential of the opposing roles of the two proteins in oral squamous cell carcinoma (OSCC). Materials and Methods Bub3 and Spindly expression was evaluated by immunohistochemistry in 62 tissue microarrays from OSCC and by real-time PCR in OSCC cell lines and in normal human oral keratinocytes. The results were analyzed as to their clinicopathological significance. RNA interference-mediated Spindly or Bub3 inhibition was combined with cisplatin treatment, and the effect on the viability of OSCC cells was assessed. Results Overexpression of Bub3 and Spindly was detected in OSCC patients. High expression of Spindly, Bub3, or both was an independent prognostic indicator for cancer-specific survival and was associated with increased cellular proliferation. Accordingly, Bub3 and Spindly were upregulated in OSCC cells comparatively to their normal counterpart. Inhibition of Bub3 or Spindly was cytotoxic to OSCC cells and enhanced their chemosensitivity to cisplatin. Conclusions The data point out Bub3 and Spindly as potential markers of proliferation and prognosis, and highlight the potential therapeutic benefit of combining their inhibition with cisplatin.
dc.description.sponsorshipCooperativa de Ensino Superior Politecnico e Universitario [AdoralLeuk-CESPU-2016, MitOralC-CESPU-2016, SpindlyTarget-CESPU-2016]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1111/odi.13089
dc.identifier.issn1354-523X
dc.identifier.issn1601-0825
dc.identifier.urihttp://hdl.handle.net/10400.1/14310
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.subjectAssembly checkpoint
dc.subjectChromosomal instability
dc.subjectDynein
dc.subjectCisplatin
dc.subjectKinetochores
dc.subjectComplex
dc.subjectCells
dc.titleSpindly and Bub3 expression in oral cancer: Prognostic and therapeutic implications
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1301
oaire.citation.issue5
oaire.citation.startPage1291
oaire.citation.titleOral Diseases
oaire.citation.volume25
person.familyNameFlorindo
person.familyNameTavares
person.givenNameClaudia
person.givenNameAlvaro
person.identifier.ciencia-id7812-FEB4-622A
person.identifier.ciencia-idAD12-B7F1-969B
person.identifier.orcid0000-0003-4549-2179
person.identifier.orcid0000-0002-7137-0944
person.identifier.scopus-author-id26531090300
person.identifier.scopus-author-id19036533500
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication4444e021-d38a-470f-b2d7-35fd2d7c1883
relation.isAuthorOfPublicationb44cba91-e2b1-4439-ba25-6ec1d68a059c
relation.isAuthorOfPublication.latestForDiscovery4444e021-d38a-470f-b2d7-35fd2d7c1883

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
odi.13089.pdf
Size:
1.07 MB
Format:
Adobe Portable Document Format
Download

Collections

FCB2-Artigos (em revistas ou actas indexadas)